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A kind of method utilizing acetic acid/sodium acetate to culture microalgae concurrently

A technology of sodium acetate and acetic acid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of fungus pollution, culture failure, algal cell death, etc., and achieves high productivity and significant economic benefits. Effect

Active Publication Date: 2021-09-07
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

Therefore, if the organic carbon source is used to carry out concurrent culture of microalgae under the condition that the aseptic culture cannot be satisfied, there will be two serious consequences: 1. The fungi (mainly bacteria) in the algae liquid will grow explosively, Severe fungal pollution occurs, ranging from inhibiting the growth and reproduction of microalgae, to causing a large number of algal cell death, resulting in failure of culture; Mass reproduction, the quality of the product cannot be guaranteed
Therefore, there is currently no way to use organic carbon for facultative trophic cultivation in large-scale cultivation systems

Method used

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  • A kind of method utilizing acetic acid/sodium acetate to culture microalgae concurrently
  • A kind of method utilizing acetic acid/sodium acetate to culture microalgae concurrently
  • A kind of method utilizing acetic acid/sodium acetate to culture microalgae concurrently

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: The influence of the timing of acetic acid addition on the number of bacteria in the co-cultured Oleococcus algae liquid

[0028] In order to achieve the purpose of using acetic acid / sodium acetate to cultivate microalgae concurrently, and at the same time control the number of bacteria in the algae liquid without fungus pollution, the inventors first studied the change law of the nitrogen source concentration of the algae liquid during the microalgae cultivation process.

[0029] Oleococcus Graesiella sp. is an oil-producing microalgae, which can synthesize a large amount of neutral lipids in cells under nitrogen stress conditions. When cultured in open ponds, the total lipid content exceeds 30%. It is a product with large-scale cultivation potential. Oil microalgae (Effective cultivation of microalgae for biofuel production: a pilot-scaleevaluation of a novel oleaginous microalga Graesiella sp. WBG-1. (2016) 9:123. DOI10.1186 / s13068-016-0541-y).

[0030]...

Embodiment 2

[0039] Embodiment 2: The influence of the timing of sodium acetate addition on the number of bacteria in the cultured Haematococcus algae liquid

[0040]Haematococcus pluvialis is a single-celled green algae, and the astaxanthin contained in its cells is a kind of carotenoid, which is the natural antioxidant with the strongest antioxidant activity found so far. (Super antioxidant activity of natural astaxanthin and its application. China Marine Medicine. 2001,20(4):45-50; Production of Haematococcus pluvialis and astaxanthin. Microalgae Biotechnology. 1999, China Light Industry Publishing House, Beijing, 174-213). Large-scale cultivation of Haematococcus to produce natural astaxanthin is an emerging microalgae biotechnology industry in the world. Astaxanthin is widely used in human health food and feed additives for precious aquatic animal breeding (large-scale cultivation of Haematococcus pluvialls to produce natural Research progress and industrialization status of astaxant...

Embodiment 3

[0050] Example 3: Acetic Acid / Sodium Acetate Facultative Nutrition Improves Biomass and Total Lipid Production in Olecoccus Graesiella sp.

[0051] The airlift photobioreactor was used to cultivate Oleococcus Graesiella sp., the composition and concentration of the medium, and the cultivation method was the same as that in Example 1. Take algae liquid samples at 8:00 am and 4:00 pm every day, observe the algae cell morphology and whether there is fungal (mainly bacteria) contamination in the algae liquid under a microscope, and measure the nitrogen source (NO 3 - )concentration. After each sampling, mark the position of the algae liquid on the outer wall of the column reactor (glass tube), and add distilled water before the next sampling to restore the liquid level of the algae liquid to the marked position, so as to replenish the water evaporated during the cultivation process.

[0052] When the nitrogen source in the algae liquid is basically consumed, the NO 3 - When th...

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Abstract

The invention discloses a method for cultivating microalgae concurrently by using acetic acid / sodium acetate. The photoautotrophic culture of microalgae is carried out by using an open or closed photobioreactor. When the nitrate concentration in the algae liquid is lower than 1.0 mg / L, add acetic acid or sodium acetate to the algae liquid, and do not add any form of nitrogen source for facultative culture. This method does not require aseptic culture operations, and is suitable for open and closed culture systems of any scale, especially solves the technical problem of using organic carbon for facultative culture in large-scale culture of microalgae. Adding Acetic acid / sodium acetate not only effectively avoids the proliferation of bacteria, but also significantly improves the yield of biomass and metabolites of microalgae.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for cultivating microalgae by using acetic acid and sodium acetate facultative nutrition in a large-scale culture system to increase the yield of biomass and secondary metabolites. [0002] technical background [0003] Microalgae resources have been widely used in human food, health products, cosmetics and other fields. The latest research shows that microalgae are expected to play an important role in human beings' response to the energy and environmental crisis caused by excessive consumption of fossil energy and excessive carbon dioxide emissions (Several scientific issues and analysis in the industrialization technology of microalgae biodiesel. Basic Science of China .2009.11(5):64-70). The production of microalgae biomass through large-scale cultivation is an important link in the utilization of microalgae resources, and the establishment of new ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 李夜光温小斌耿亚洪陶焕平丁奕王中杰
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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