Minimally-invasive detection kit for serum miRNA combination

A technology of serum and mir-4286, which is applied in the field of serum marker detection, can solve the problems of low content and inability to accurately predict the prognosis of patients, and achieve the effects of high specificity, fast and convenient detection, and high accuracy

Active Publication Date: 2018-09-28
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0005] At present, most of the same type of marker kits are mainly used to detect tissue samples, and they are invasive tests.
Moreover, the detection molecules of the same type of serum are usually proteins, which are macromolecular substances that are easily degraded, have low content, and are limited by detection methods, and cannot accurately predict the prognosis of patients.

Method used

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  • Minimally-invasive detection kit for serum miRNA combination
  • Minimally-invasive detection kit for serum miRNA combination
  • Minimally-invasive detection kit for serum miRNA combination

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Embodiment

[0062] In this example, the miRNA chip results of 25 patients with oral squamous cell carcinoma patients with complete follow-up data of more than four years were counted, and the median of the standardized value of each miRNA was used as the cutoff value, and the chip values ​​greater than this cutoff value corresponded to The patients with high expression group are defined as the high expression group, and the patients corresponding to the microarray value less than or equal to this cutoff value are defined as the low expression group. The corresponding cutoff value is calculated for each miRNA, and the statistical analysis of the survival curve is performed on the high and low expression groups divided into these groups. , the specific miRNA that can specifically distinguish the prognosis of patients with oral squamous cell carcinoma after surgery, that is, the survival curve, was obtained, which was statistically significant (p<0.05). details as follows:

[0063] (1) Based...

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Abstract

The invention provides a minimally-invasive detection kit for a serum miRNA combination. The kit uses miR-626, miR-1537-3p, miR-4286 and miR-5100 as molecular markers for oral squamous cell carcinomain a combined mode, and the joint expression level of the individual miR-626, miR-1537-3p, miR-4286 and miR-5100 is judged in a curve-fitting mode. The minimally-invasive detection kit is rapid in detection, convenient to detect, high in detection sensitivity and specificity, low in cost, capable of meeting the detection needs of most cancer patients and extremely wide in application range, and the forecast accuracy is high through clinical validation.

Description

technical field [0001] The invention belongs to the technical field of serum marker detection and relates to a minimally invasive detection kit for serum miRNA combination. Background technique [0002] Although miRNA has unique advantages as a biomolecular marker, there are also some technical problems. If the sample size is larger and the number of independent detection cohorts is larger, the accuracy of miRNA prediction will be higher, and it will be closer to the characteristics of the tumor population as a whole. Samples are not easy to collect, and the workload of sample preservation and recording is heavy. [0003] At present, the mainstream method for screening candidate marker miRNA molecules is generally a high-throughput method for initial screening, and then real-time polymerase chain reaction for verification. Although the method is stable and technically mature, it is also prone to some false positive results. Using the probe method to detect candidate miRNA...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/118
Inventor 徐骎石剑波鲍欣刘增赢陈万涛
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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