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The ssr primers of Robinia pseudoacacia leaf gall midge and its application in population genetic diversity analysis

A technology of genetic diversity and S. larvae, which is applied to the specific SSR primers of the locust leaf gall mosquito S. spp. and its application field, can solve the problems of origin, spread and genetic differentiation, etc., which are still blank.

Active Publication Date: 2021-12-07
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the research on the locust leaf gall midge is still in the stage of basic biological research, and its origin, spread and genetic differentiation are still blank

Method used

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  • The ssr primers of Robinia pseudoacacia leaf gall midge and its application in population genetic diversity analysis
  • The ssr primers of Robinia pseudoacacia leaf gall midge and its application in population genetic diversity analysis
  • The ssr primers of Robinia pseudoacacia leaf gall midge and its application in population genetic diversity analysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Design and screening of embodiment 1 specific SSR primer

[0057] 1 test material

[0058] Collect the damaged leaves of Robinia pseudoacacia in the wild and send them back to the laboratory, put them into jars for rearing, cover the mouth of the jar with three layers of gauze, and tie it tightly with rubber bands to prevent the bees from emerging and escaping; constant temperature (25°C) ) and constant humidity (70%), managed by full-time personnel, put the eclosion adults into centrifuge tubes containing absolute ethanol with a small brush every day, and store them at -20°C for later use.

[0059] 2 test method

[0060] 2.1 DNA extraction

[0061] The adults of Robinia pseudoacacia leaf gall midge, Leptospermia grandis, in Yingkou City, Liaoning Province were taken as the object, and the genomic DNA was extracted by referring to Wang Wen and Shi Liming (1993), and the collected genomic DNA was detected by 1% agarose gel electrophoresis.

[0062] 2.2 Genomic DNA Fra...

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Abstract

The invention discloses an SSR primer of the locust leaf gall midge and its application in population genetic diversity analysis. The present invention designs and obtains more than 130,000 SSR sequences based on the mitochondrial genome data of the locust leaf gall midge, A. spp., and screens out 37 pairs of SSR-specific primers with relatively stable differences and good polymorphism. The present invention further discloses the genetic diversity analysis, population evolution analysis, and polymorphism analysis of the 37 pairs of SSR-specific primers in the population of Robinia pseudoacacia leaf gall midge and the construction of the fingerprint of Robinia pseudoacacia leaf gall midge and other applications. The invention has important value for carrying out research on the genetic diversity of natural enemy groups such as the locust locust leaf gall midge and the population differentiation of the population, and grasping its source and population differentiation.

Description

technical field [0001] The present invention relates to SSR primers, in particular to specific SSR primers for Platygaster robiniae Buhl and Duso, and the present invention further relates to the genetic diversity of the specific SSR primers in the population of Robinia robiniae Buhl and Duso It belongs to the field of specific SSR primers of Robinia locust leaf gall midge and its application in aspects such as sex analysis. Background technique [0002] Platygaster robiniae Buhl and Duso (Hymenoptera: Platygastridae) is an important alien species in China, Obolodiplosis robiniae (Haldeman) (Diptera: Gastrophyidae) egg-larva intertemporal parasitic natural enemy, The parasitism rate of Robinia pseudoacacia leaf gall midge is relatively high, especially in the later stage of its host year, the parasitism rate can often reach more than 80%, and it is a very good natural enemy resource. In the past ten years, the host of the locust leaf gall midge, Robinia pseudoacacia leaf ga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/156C12Q2525/151
Inventor 姚艳霞杨君赵文霞淮稳霞林若竹
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY