Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A breast cancer 21 gene detection kit and detection method thereof

A gene detection and kit technology, applied in the field of genes, can solve the problems of poor specificity and sensitivity, complicated operation, etc.

Active Publication Date: 2018-12-18
MICROPROBE MEDICAL TECH SHANGHAI CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a breast cancer 21 gene detection kit and its detection method in order to overcome the defects of poor specificity, sensitivity or complicated operation in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A breast cancer 21 gene detection kit and detection method thereof
  • A breast cancer 21 gene detection kit and detection method thereof
  • A breast cancer 21 gene detection kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0203] , Extraction of RNA from paraffin samples

[0204] After extracting the RNA of the breast cancer sample, the sample A to be tested is obtained;

[0205] 1) Put the breast cancer sample into a 1.5ml centrifuge tube;

[0206] 2) Add 240ml PKD Buffer, shake and mix, and centrifuge at 11000g for 1min;

[0207] 3) Add 10ul proteinase K, pipette gently to mix;

[0208] 4) Incubate at 56°C for 15 minutes, then at 80°C for 15 minutes;

[0209] 5) Incubate on ice for 3 minutes, centrifuge at 20000g for 15 minutes;

[0210] 6) Transfer the upper liquid to a new 1.5ml centrifuge tube;

[0211] 7) Add 25ul of DNase Booster Buffer (about 1 / 10 of the liquid in the centrifuge tube), and 10ul of DNaseI stock solution, mix upside down and centrifuge briefly to mix the liquid on the tube wall into the bottom;

[0212] 8) Incubate at room temperature for 15 minutes;

[0213] 9) Add 500ul Buffer RBC to adjust the binding environment and mix well;

[0214] 10) Add 1200ul absolute eth...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a breast cancer 21 gene detection kit and a detection method thereof. The kit comprises primers and probes for detecting genes ACTB, GAPDH, RPLPO, GUS, TFRC, CD68, BAG1, GSTM1,HER2, GRB7, MMP11, CTSL2, KI67, STK15, CCNB1, MYBL2, Survivin, ESR1, PGR, BCL2 and SCUBE2. Two pairs of primers and two specific probes are respectively designed for 21 genes in the kit, a primer andprobe liquid of each gene is placed in one reaction tube, a same buffer solution and a procedure are adopted for QPCR (Quantitative Polymerase Chain Reaction) amplification, the operation is simple and convenient to carry out, the detection time can be shortened, and clear and accurate results can be achieved; the kit is wide in application, and applicable to both scientific research and clinicaldetection and applicable to single or multiple samples, and rapid and accurate detection results can be achieved.

Description

technical field [0001] The invention relates to the field of gene technology, in particular to a breast cancer 21 gene detection kit and a detection method thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. In the past 20 years, the multidisciplinary comprehensive treatment model centered on surgery and assisted by chemotherapy, radiotherapy, and endocrine therapy has been greatly developed, which has significantly increased the recurrence and mortality of breast cancer. However, there is still a lack of effective methods that can accurately predict the recurrence risk of patients and give corresponding treatment. [0003] The breast cancer recurrence risk assessment system is based on RT-QPCR method to detect the expression of 16 tumor-related target genes in tumor tissue through precise calculation, that is, breast cancer 21 gene detection, and the breast cancer risk is obtained by calculating the amount of gene expres...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118
Inventor 唐荣苏丽
Owner MICROPROBE MEDICAL TECH SHANGHAI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com