Fixed wavelength Raman scattering fast acquisition and imaging device

A technology of Raman scattering and fixed wavelength, which is applied in the field of Raman scattering fast acquisition and imaging equipment, which can solve the problems of Raman signal weakening, spectrum acquisition and imaging speed slowdown, etc.

Active Publication Date: 2020-07-07
上海氘峰医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing Raman spectroscopy technology can fully reflect the Raman signal intensity distribution of the measured sample at different wavelengths, but the disadvantage is that the Raman signal is weakened with the grating spectroscopy, and the resulting spectrum acquisition and imaging speed are slowed down.

Method used

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  • Fixed wavelength Raman scattering fast acquisition and imaging device
  • Fixed wavelength Raman scattering fast acquisition and imaging device
  • Fixed wavelength Raman scattering fast acquisition and imaging device

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Experimental program
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Effect test

Embodiment

[0075] refer to figure 1 A fixed-wavelength Raman scattering fast acquisition and imaging device includes a laser emitting unit, a mirror group, a Raman scattering signal filtering unit, and a Raman scattering signal acquisition unit. The laser emitting unit is used to obtain the laser with adjusted power, and the mirror group is used to reflect the laser with adjusted power to the Raman scattering signal filtering unit, and the Raman scattering signal filtering unit is used to make the laser with adjusted power hit the surface of the sample Raman scattering occurs, and the Rayleigh scattering signal scattered by the sample is blocked, and the Raman scattering signal scattered by the sample is transmitted. The Raman scattering signal acquisition unit is used to receive the Raman scattering signal. The Raman scattering signal acquisition unit includes a beam splitter 12 , the beam splitter 12 is used to split the Raman scattering signal into two strands, which are respectively...

Embodiment 2

[0084] Single-cell Raman spectrum acquisition of deuterium-absorbed Escherichia coli:

[0085] A single clone of E.coli (preservation number ATCC25922, purchased from ATCC) was picked from the plate, inoculated into 5ml LB liquid medium, and cultured overnight in a constant temperature incubator (37°C, 150rpm). Transfer the overnight cultured bacteria to 5 ml containing different concentrations of D ranging from 0% to 50% at a ratio of 1:1000. 2 O LB liquid medium, incubated in a constant temperature incubator (37°C, 150rpm) for 4h. Take 1ml of the bacterial solution, centrifuge at 5000rpm for 2min to remove the supernatant, add 1ml of sterile water, pipette and pipette 3 to 5 times, centrifuge at 5000rpm for 2min to remove the supernatant, repeat the above steps of adding water and washing once. Finally, add 1ml of sterile water, pipette the gun to mix the bacterial solution, and then start the spotting test. When spotting, take 1 μl of the sample onto a calcium fluoride gl...

Embodiment 3

[0088] Adopt the fixed-wavelength Raman scattering rapid acquisition and imaging equipment of the present invention, the laser 1 selects a 532nm laser with adjustable power, and the laser narrowband filter 2 selects 532 / 2nm, that is, the transmittance at 532nm is greater than 90%, and the transmittance is greater than 90%. The full width at half maximum of the curve is 2nm to filter out the stray lines in the laser.

[0089] The beam splitter 12 is a 10:90 beam splitter. After 90% of the Raman signal penetrates the beam splitter 12, it is read by the first detector 14 after passing through the first narrow-band filter 13, and 10% of the Raman signal is read by the first detector 14. After being reflected by the beam splitter 12 , it is read by the second detector 16 after passing through the second narrow-band filter 15 .

[0090] The working range of the beam splitter 12 is 400-1000nm, and the first narrow-band filter 13 selects 600 / 14nm, that is, the central wavelength is 60...

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Abstract

Provided is a fixed-wavelength Raman scattering quick collection and imaging device, which comprises a laser emission unit (1), mirror groups (4, 5), Raman scattered signal filtering units (6, 13, 15) and Raman scattered signal collection units (14, 16), wherein the laser emission unit (1) is used for obtaining a power regulated laser; the reflector groups (4, 5) are used for reflecting the power regulated laser to a surface of a sample; the Raman scattered signal filtering units (6, 13, 15) are used for dividing a Raman scattered signal into a target signal and background noise, and narrowing the wavelength ranges of the target signal and the background noise respectively; and the Raman scattered signal collection units (14, 16) are used for collecting the two Raman signals. Compared with the prior art, light splitting by an optical grating is omitted in the device, instead, the optical filters (6, 13, 15) are used for filtering the signal range of a target wavelength, and a photon counter or a photomultiplier tube is used to collect signals, so that the intensity of Raman signals of a sample is greatly improved, the collection time is shortened, and quick imaging is realized. Optical elements such as optical gratings are omitted, and the construction cost is much lower than that of a traditional Raman product in the market.

Description

technical field [0001] The invention relates to a Raman imaging device, in particular to a fixed-wavelength Raman scattering fast acquisition and imaging device. Background technique [0002] Chinese patent CN107462566A discloses a Raman spectrometer for detecting a specific narrow wavenumber range. The Raman spectrometer includes a laser emitting device, a spectrum acquisition device, and a data processing device. The laser emitting device is used to obtain a power-adjusted excitation beam, so The spectrum acquisition device is used to make the power-adjusted excitation beam hit the surface of the sample to cause Raman scattering, and filter out the directional Rayleigh scattered light to obtain directional Raman scattered light, and make the directional Raman scattered light be received by the receiver. The data processing device is a computer device connected to the receiver for the formation, analysis and judgment of the obtained Raman spectrum; the receiver is a single-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 黄巍黄霞宋一之徐嘉宝衣晓飞彭迪
Owner 上海氘峰医疗科技有限公司
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