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Hollow gold nanoparticle-DNA composite base and preparation method thereof

A gold nanoparticle, composite substrate technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of different adsorption properties, quantitative analysis limitations, etc., to achieve simple preparation method, good chemical stability, and increased Raman scattering intensity. Effect

Inactive Publication Date: 2015-04-29
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many limiting factors in SERS technology. It requires the sample to be in contact with the substrate, which loses the advantages of non-invasive and non-contact Raman spectroscopy; its substrate has different adsorption properties for different trace molecular materials, making quantitative analysis is limited

Method used

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  • Hollow gold nanoparticle-DNA composite base and preparation method thereof
  • Hollow gold nanoparticle-DNA composite base and preparation method thereof
  • Hollow gold nanoparticle-DNA composite base and preparation method thereof

Examples

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preparation example Construction

[0034] A method for preparing a hollow gold nanoparticle-DNA composite substrate, the method comprising the following steps:

[0035] (1) Design the probeDNA single strand shown in SEQ ID NO.1 and the reporterDNA single strand shown in SEQ ID NO.2.

[0036] probeDNA is 5'-acatagcacattcgaggtag-3';

[0037] reporterDNA is 5'-ctacctcgaatgtgctatgt-3';

[0038] (2) Assembling a first hollow gold nanolayer with a uniform and dense monolayer on the Si substrate;

[0039] Firstly, hollow gold nanospheres are prepared, which can be proposed by Kobayashi et al. (Y. Kobayashi; M. Horie; M. Konno; B.R. Gonzalez; L. Marzan, J. Phys. Chem. B, 2003(107), 7420.) method, or proposed by L.J. Wan et al. (H.P. Liang; L.J. Wan; C.L. Bai; L. Jiang; Gold Hollow Nanospheres: Tunable Surface Plasmon Resonance Controlled by Interior-Cavity Sizes. J. Phys. Chem. B 2005(109), 7795-7800) method to prepare hollow gold nanoparticles with different sizes and wall thicknesses, and the preparation process i...

Embodiment 1

[0048]In this example, a single-layer hollow gold nanoparticle-DNA layer structure, ie, a Si / HGN-DNA structure, was prepared, and a Raman activity test was performed.

[0049] Design samples Si / HGN-probeDNA-probeDNA (non-complementary paired DNA single strand) and Si / HGN-probeDNA-reporterDNA (complementary paired DNA single strand) structures.

[0050] Saltify probeDNA and reporterDNA separately: Mix 900μL of PBS solution with pH=7.1, 100μL of 0.1% sodium dodecyl sulfate and 10μL of 1mmol / LprobeDNA (or 10μL of 1mmol / L reporterDNA), and let the mixed solution stand for 6h followed by salinization. The salinization process is as follows: add equal volumes of NaCl solution with increasing concentration to the mixed solution in 5 times, and add 100 μL of NaCl solution each time; the concentration of NaCl solution in the first drop is 2mol / L, and the concentration gradient of NaCl solution is 0.05mol / L; after adding NaCl solution for 5 times, the NaCl concentration in the final s...

Embodiment 2

[0054] In this example, a double-layer hollow gold nanoparticle layer is prepared, and the interlayer is a composite material of a DNA layer, that is, a Si / HGN-DNA-HGN structure, and a Raman activity test is performed:

[0055] Connect reporterDNA to hollow gold nanoparticles in hydrosol: mix 500 μL of PBS solution with pH=7.1, 100 μL of 0.1% sodium dodecyl sulfate, 10 μL of 1 mmol / L reporterDNA and 400 μL of gold hollow sphere hydrosol, and statically Set aside for 6h to carry out salinization. The salinization process is as follows: add equal volumes of NaCl solution with increasing concentration to the mixed solution in 5 times, and add 100 μL of NaCl solution each time; the concentration of NaCl solution in the first drop is 2mol / L, and the concentration gradient of NaCl solution is 0.05mol / L; after adding NaCl solution for 5 times, the NaCl concentration in the final solution was 0.7mol / L. The mixture was left to stand for 24 hours and then centrifuged at a speed of 700...

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Abstract

The invention discloses a hollow gold nanoparticle-DNA composite base and a preparation method thereof. The composite base disclosed by the invention is composed of a first hollow gold nano layer, a DNA layer and a second hollow gold nano layer. Due to the structure, a coupling effect of surface plasmon between the metal nanoparticles exists in the horizontal direction; coupling of the surface plasmon is also promoted in the direction vertical to the base so as to form relatively many hot spots; and a DNA chain is located in a hot spot coupling area generated between two hollow gold nanoparticle layers, so that the raman scattering intensity of the DNA is significantly enhanced; and compared with a traditional raman active substrate, the composition base prepared by the method has great advantages in enhancement of the raman scattering intensity.

Description

technical field [0001] The invention relates to a hollow gold nanoparticle-DNA composite substrate and a preparation method thereof: a single-layer hollow gold nanoparticle film is prepared by using interface self-assembly technology, and the upper and lower layers of hollow gold nanoparticle The particle layers are assembled together to prepare a hollow gold nanoparticle layer-DNA layer-hollow gold nanoparticle layer composite substrate as a surface-enhanced Raman substrate. Background technique [0002] Surface-enhanced Raman scattering (SERS) spectroscopy is a very effective tool for detecting interface properties and intermolecular interactions, and characterizing surface molecular adsorption behavior and molecular structure. Based on its single-molecule level sensitivity and molecular specificity, This technique has become one of the most sensitive techniques for studying interface effects, and is widely used in the study of the orientation and adsorption behavior of ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 刘爱萍汤建吉艺昕
Owner ZHEJIANG SCI-TECH UNIV
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