Ridge-shaped magnetic frame for extracting nucleic acid by paramagnetic particle method

Abstract

The invention discloses a ridge-shaped magnetic frame for extracting nucleic acid by a paramagnetic particle method. The ridge-shaped magnetic frame includes an upper plate, a vertical plate and a bottom plate, wherein the vertical plate is vertically fixed to the position of the symmetric line of the bottom plate, and the upper plate is fixed to both sides of the top end of the vertical plate. Tension holes are formed in the upper plate, expandable tension rings are arranged below the tension holes, and magnets are arranged on both sides of the vertical plate. A plurality of positioning holesare formed in the corresponding positions, below the tension holes, on the bottom plate, the positioning holes are located on both sides of the vertical plate, and the number of the positioning holesis the same as that of the tension holes. The ridge-shaped magnetic frame for extracting the nucleic acid by the paramagnetic particle method has the advantages that centrifuge tubes cannot rotate orfall off during the movement of the magnetic frame, and in the operation of removing supernatant waste liquid after magnetic beads attract, the magnetic frame can be inverted and the centrifuge tubescannot fall off; the ridge-shaped upper plate enables covers of the centrifuge tubes on both sides to be far away from each other, so that sample holes on both sides are convenient to use for operation at the same time, the effective flux increases, and moreover, the distance and orientation also effectively reduce the cross-contamination of sample tubes located in the holes on both sides duringthe cover opening operation.

Description

technical field [0001] The invention relates to the field of experimental equipment, in particular to a roof-shaped magnetic frame for extracting nucleic acid by a magnetic bead method. Background technique [0002] At present, the magnetic bead method is often used to extract sample nucleic acid in biomolecular experiments, and the container often used in the experiment is a 1.5 to 2ml eppendorf centrifuge tube. The upper end of the centrifuge tube is cylindrical and the lower end is conical. This shape feature is conducive to more efficient manipulation of small volumes of liquid, so it is widely used in biomolecular experiments. The structure design of the existing magnetic frame is unscientific: the current design idea of ​​the magnetic frame is to make the sample hole as close as possible to the middle magnet, the purpose is to make the eppendorf centrifuge tube closer to the magnet, which is conducive to adsorption, but it brings two problems: ( 1) Since the bottom of...

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Problems solved by technology

The structure design of the existing magnetic frame is unscientific: the current design idea of ​​the magnetic frame is to make the sample hole as close as possible to the middle magnet, the purpose is to make the eppendorf centrifuge tube closer to the magnet, which is conducive to adsorption, but it brings two problems: ( 1) Since the bottom of the centrifuge tube is tapered, when the upper main part of the centrifuge tube is close to the magnet, its conical lower part is relatively far away, which is not conducive to the adsorption of magnetic beads at the bottom of the centrifuge tube; (2) Since the centrifuge tube distance is close Too close to form a space barrier and easy to form cross-contamination

Moreover, it is often not fixed on the magnetic stand or is not easy to fix on the magnetic stand. A little carelessness during operation may cause the centrifuge tube to move and fall off, resulting in the magnetic beads not being completely adsorbed in place during the nucleic acid extraction operation, thereby affecting the experimental results.

The more obvious impact is that the operation of extracting nucleic acid by the magnetic bead method often needs to be reversed. When it is inverted, the centrifuge tube will fall if it is not fixed on the magnetic stand.

In addition, the existing magnetic frame adopts the design that the magnet is located in the middle vertical plate, and the centrifuge tube holes are distributed on both sides of the vertical plate and are closely adjacent to each other, so that the centrifuge tube is as close as possible to the magnet, but it brings a defect: the distance between the two rows of holes is too large Close to each other in space, the two adjacent pipe covers fight, so the two rows of holes on both sides of the neutral plate cannot be used at the same time in actual operation, wasting resources and reducing efficiency

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