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Application of TOP2A (Topoisomerase(DNA)II Alpha) autoantibody as lung cancer diagnostic marker

An autoantibody, lung cancer diagnosis technology, applied in the field of bioengineering, can solve the problem of unsatisfactory tumor markers, and achieve the effects of low cost, high accuracy and simple operation

Inactive Publication Date: 2018-10-12
ZHENGZHOU UNIV
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Problems solved by technology

[0008] The purpose of the present invention is to overcome the problem of unsatisfactory existing tumor markers of lung cancer, provide a new application of TOP2A, and improve the sensitivity, specificity and accuracy of early diagnosis of lung cancer

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  • Application of TOP2A (Topoisomerase(DNA)II Alpha) autoantibody as lung cancer diagnostic marker
  • Application of TOP2A (Topoisomerase(DNA)II Alpha) autoantibody as lung cancer diagnostic marker
  • Application of TOP2A (Topoisomerase(DNA)II Alpha) autoantibody as lung cancer diagnostic marker

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Embodiment Construction

[0028] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0029] The present invention adopts pre-recombined cDNA expression library serological analysis technology (SEREX) to screen antigens that may be related to lung cancer, utilizes three independent cohort studies of Oncomine database to carry out further mining, and uses ELISA method to detect the TOP2A antibody level in the serum of lung cancer patients (see figure 1 ).

[0030] 1) For the antigen TOP2A that may be associated with lung cancer screened by the previous SEREX method, three independent cohort studies (including 375 cases of non-small cell lung cancer and 134 cases of normal tissue) in the Oncomine database were used to further mine, and the TOP2A was analyzed in lung cancer and lung cancer. mRNA expression in normal tissues.

[0031] 2) In order to verify whether the TOP2A autoantibody can be us...

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Abstract

The invention discloses an application of TOP2A (Topoisomerase(DNA)II Alpha) autoantibody as a lung cancer diagnostic marker, and further discloses a screening and identification method for the TOP2Aautoantibody as the lung cancer diagnostic marker. A serum biomarker which is used for clinical lung cancer diagnosis and is simple to operate, low in cost, high in accuracy and noninvasive is provided. Research shows that with application of an ELISA (enzyme-linked immunosorbent assay) method in detection of the serum TOP2A autoantibody, lung cancer patients, normal people and patients with chronic lung diseases can be more accurately distinguished, and the TOP2A autoantibody can be used for early diagnosis of lung cancer. Under the background, the lung cancer patients are detected conveniently, quickly and effectively, and the TOP2A autoantibody can be used for clinical early diagnosis of lung cancer.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to the use of TOP2A autoantibody as a lung cancer diagnostic marker for early diagnosis of lung cancer. Background technique [0002] Lung cancer is a malignant tumor with the highest morbidity and mortality in China and the world. The clinical manifestations of lung cancer are complex and diverse, and the manifestations of early lung cancer are insidious and diverse. The clinical features and signs are not obvious. They only manifest as non-specific symptoms such as cough, expectoration, chest pain, fever, and physical decline, without timely treatment. Therefore, most lung cancer patients are diagnosed at an advanced stage. According to clinical data, nearly 60% of patients are already in the advanced stage of lung cancer when they see a doctor. The 5-year survival rate of advanced lung cancer is only about 16%, while the 5-year survival rate of early stage lung cancer (sta...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 代丽萍王鹏张建营裴露王婷婷王凯娟宋春花叶华王晓史健翔
Owner ZHENGZHOU UNIV
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