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Lignin-derived tyrosinase inhibitor and preparation method thereof

A technology of tyrosinase and lignin, which is applied in the field of high-value lignin utilization, and can solve problems such as high dispersion, uneven structure, and uneven lignin activity

Active Publication Date: 2018-10-16
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to prepare a high-efficiency tyrosinase inhibitor derived from lignin. At the same time, aiming at the problem of inhomogeneous lignin activity caused by inhomogeneous lignin structure and high dispersion, the use of dichloromethane to extract small molecular weight Preparation of high-efficiency lignin-derived tyrosinase inhibitors by lignin method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The air-dried corn stalks were pulverized, and 50% ethanol aqueous solution (v / v) was added to cook at 150° C. for 1 h according to the solid-to-liquid ratio of 1:12 (m / v). After the cooking liquor was obtained by suction filtration, ethanol was removed by rotary evaporation and lignin was precipitated. The precipitated lignin is washed with water and centrifuged to remove water-soluble impurities, and freeze-dried to obtain ethanol lignin. After mixing corn stalk ethanol lignin and dichloromethane at a solid-to-liquid ratio of 1:40 (m / v), the lignin raw material was fully dissolved in dichloromethane by ultrasonication for 0.5 h, and the supernatant was obtained by centrifugation. The supernatant was rotary evaporated to remove dichloromethane to obtain low molecular weight lignin. The obtained low molecular weight lignin was analyzed for tyrosinase inhibitory activity, and at a concentration of 0.4 mg / ml, the inhibitory rate of tyrosinase activity was 70.56%.

Embodiment 2

[0014] The air-dried corn stalks were crushed, and 60% ethanol aqueous solution (v / v) was added to cook at 160° C. for 2 hours according to the solid-to-liquid ratio of 1:10 (m / v). After the cooking liquor was obtained by suction filtration, ethanol was removed by rotary evaporation and lignin was precipitated. The precipitated lignin is washed with water and centrifuged to remove water-soluble impurities, and freeze-dried to obtain ethanol lignin. After mixing corn stalk ethanol lignin and dichloromethane at a solid-to-liquid ratio of 1:45 (m / v), the lignin raw material was fully dissolved in dichloromethane by ultrasonication for 1 h, and the supernatant was obtained by centrifugation. The clear liquid was removed by rotary evaporation to remove dichloromethane to obtain low molecular weight lignin. The obtained small molecular weight lignin was analyzed for tyrosinase inhibitory activity, and at a concentration of 0.4 mg / ml, the inhibitory rate of tyrosinase activity was 7...

Embodiment 3

[0016] The air-dried corn stalks were crushed, and 80% ethanol aqueous solution (v / v) was added to cook at 160° C. for 2 hours according to the solid-to-liquid ratio of 1:15 (m / v). After the cooking liquor was obtained by suction filtration, ethanol was removed by rotary evaporation and lignin was precipitated. The precipitated lignin is washed with water and centrifuged to remove water-soluble impurities, and freeze-dried to obtain ethanol lignin. After mixing corn stalk ethanol lignin and dichloromethane at a solid-to-liquid ratio of 1:50 (m / v), the lignin raw material was fully dissolved in dichloromethane by ultrasonication for 0.5 h, and the supernatant was obtained by centrifugation. The supernatant was rotary evaporated to remove dichloromethane to obtain low molecular weight lignin. The obtained low molecular weight lignin was analyzed for tyrosinase inhibitory activity, and at a concentration of 0.4 mg / ml, the inhibitory rate of tyrosinase activity was 73.12%.

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Abstract

The invention belongs to the field of high-value utilization of lignin and mainly relates to an application of small-molecule lignin as a tyrosinase inhibitor. Aiming at the problem of non-uniform lignin activity caused by non-uniform lignin structure and high molecular weight dispersion degree, the efficient lignin-derived tyrosinase inhibitor is prepared with the method for obtaining small-molecule lignin by dichloromethane grading. The method comprises the following steps: corn stalk is taken as a raw material and cooked at high temperature with ethanol water, ethanol lignin is extracted, ethanol is removed by reduced pressure distillation, ethanol lignin is obtained by freeze drying and graded by dichloromethane, and small-molecule lignin with high tyrosinase inhibition activity is obtained. By taking lignin-derived small-molecule phenolic compound as a natural tyrosinase inhibitor, use value of lignin is improved, and a novel cheap source is provided for the natural tyrosinase inhibitor.

Description

technical field [0001] The invention belongs to the field of high-value utilization of lignin, and mainly relates to the application of small molecule lignin as a tyrosinase inhibitor. Background technique [0002] Lignin is an aromatic polymer compound, mainly found in plant fibers, and is one of the components of plant cell walls. The content of lignin in plants is second only to cellulose, accounting for about 25%, and it is an important renewable resource. The main source of lignin is industrial pulping waste liquid. The pulp and paper industry can separate a large amount of lignin from wood every year, but its effective utilization rate is very low. Most lignin is burned to provide electricity and heat. As a natural aromatic compound, lignin contains various functional groups such as phenolic hydroxyl group, aldehyde group and carboxyl group in its structure, and it is an important source that can replace petroleum-based chemicals, especially aromatic compounds. Ligni...

Claims

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Application Information

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IPC IPC(8): C07G1/00A61P17/00
CPCA61P17/00C07G1/00
Inventor 王冠华司传领王蕊
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY