Feature sequence of carya illinoensis variety Davis, marker primer and identification method

Abstract

The invention relates to a feature sequence of a high-specificity carya illinoensis variety Davis, a molecular specificity marker primer and a method which can carry out rapid identification on the carya illinoensis variety Davis. The molecular specificity marker primer has the following sequence that: an upstream primer: 5'-TCCTGAAAGCAGCCACAACA-3'; a downstream primer: 5'-GACATGTGTACGAGGTGGTCA-3'. The molecular specificity marker primer disclosed by the invention can carry out rapid early identification on the carya illinoensis variety Davis; the method is simple, rapid, convenient and accurate and is molecular means which cannot be replaced by a mode of distinguishing the carya illinoensis variety by appearance features.

Description

technical field [0001] The invention relates to a characteristic sequence of the pecan variety Davis, a molecular specific marker primer and a method for quickly identifying the pecan variety Davis by using the molecular specific marker primer. Background technique [0002] Thin shell hickory (Carya illinoënsis (Wangenh.) K.Koch) is the most economically valuable tree species in the genus Hickory in the family Juglandaceae. Carya carya is a typical outcrossing plant. According to the existing production practice, the variety configuration of carya carya is one of the main factors affecting its yield. The U.S. is one of the origins of pecans and its central production area. Over the years, the U.S. has selected and named about 1,000 cultivars. It has been more than 100 years since the introduction of hickory nuts in my country, and there are dozens of varieties commonly used in production. Years of production practice have proved that the vast subtropical regions of my count...

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Problems solved by technology

[0003] However, the main problems faced by the existing pecan production areas in my country are low and unstable yields.

There are many reasons for this phenomenon, one of which is the lack of clear genetic relationship analysis of the existing introduced varieties, coupled with the confusion in the naming of some hybrid offspring, it is difficult to carry out effective parent selection and rational allocation, and it is not convenient for variety identification , promotion, exchange and cultivation of new varieties, so developing stable and specific DNA fingerprints for some varieties at the molecular level is the scientific way to achieve accurate and rapid identification of pecan varieties;

[0004] Some identification and genetic relationship analysis methods of pecan varieties based on SSR molecular markers have been reported at home and abroad, but the detection is relatively cumbersome and the results are unstable.

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