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A polysaccharide-producing space Hericium coralii st21-2 and its application in improving biological antioxidant capacity
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A Hericium erinaceus, coral-like technology, applied in the polysaccharide-producing space Hericium erinaceus ST21-2 and its application in improving biological anti-oxidation, can solve the problem that the development of microorganisms in food space is still in its infancy, space microorganisms Stay and other issues
Active Publication Date: 2021-09-14
FULLARTON BIOENG TECH BEIJING CO LTD
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[0003] At present, domestic and foreign scholars' research on Hericium erinaceus mainly focuses on the optimization of the extraction conditions of polysaccharides and the analysis of the structure of the substances contained in them. For space pathogens, saprophytes and pharmaceutical applications, the development of food space microbes is still in its infancy
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Embodiment 1
[0033] Embodiment 1, the screening of Hericium erinaceus in space
[0034] 1. Space mutagenesis of bacterial strains
[0035] The ground Hericium erinaceus GT21 was carried back by Tiangong 2 and Shenzhou 11 spacecraft, and several strains of space mutagenesis were obtained.
[0036] 2. Activation of strains
[0037] Take the space-mutated strains frozen in the ultra-low temperature refrigerator at -80°C and the preserved strains of Hericium erinaceus GT21 glycerol tubes, dip a small amount of bacterial solution with the inoculation loop and inoculate them on the slant of the YEPD medium test tube, and incubate at 28°C for 6 days , used for subsequent experiments after continuous activation for 3 generations.
[0038] 3. Expansion of strains
[0039] The space-mutated strain activated for 3 generations in step 2 and Hericium erinaceus GT21 were inoculated on the slant of the Erlenmeyer flask of YEPD medium, and cultured at 28°C for 6 days.
[0073] Inoculate Hericium coralliformus ST21-2 into the seed fermentation medium, culture at 28°C, shake at 160r / min for 12 days, press 8 times / sec with a sterile homogenizer, beat for 1min, beat the mycelial balls, press 10 The inoculum of % (volume percentage) is moved into the seed fermentation medium, 28 ℃, 160r / min shaking culture 5 days, get fermentation system, filter with three layers of sterile gauze, collect mycelium, the mycelium that obtains is used without After washing with distilled water for 3-4 times, freeze-dry in vacuum.
[0074] 2. Preparation of mycelium crude polysaccharides
[0075] Grind the freeze-dried mycelia in step 1 into powder, weigh 1 g of the sample, add 18 mL of distilled water, extract in a boiling water bath for 2 hours, filter with three layers of gauze, repeat the extraction once with the filter residue, combine the...
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Abstract
The invention discloses a polysaccharide-producing space coralliform Hericium ST21‑2 and its application in improving biological antioxidation. The Hericium coralloides Fullarton-H-ST21-2 provided by the present invention has a preservation number of CGMCC No. 15378 in the General Microorganism Center of China Committee for Microorganism Culture Collection. The present invention adopts Hericium erinaceus carried by Tiangong 2 and Shenzhou 11 spaceships back to the ground and undergoes space mutation, and uses the original Hericium erinaceus on the ground as a control to select a strain with higher antioxidant activity, which is the space monkey. The application of polysaccharides from Mycelium cephalosporins in improving antioxidant activity provides a practical basis. At the same time, to a certain extent, it broadens the scope of space microbial research and fills the gap in the research and development of fungal microbial functions in food space.
Description
technical field [0001] The invention relates to a polysaccharide-producing space coral-shaped Hericium ST21-2 and its application in improving biological anti-oxidation. Background technique [0002] Hericium erinaceus polysaccharides (HEP) are mainly composed of glucose, galactose, and mannose, and are composed of main chains connected by β-(1,3) bonds and branched chains connected by β-(1,6) bonds. It is a kind of glucan, and it is also an important functional active substance produced by the fermentation of Hericium erinaceus. Hericium erinaceus polysaccharides include polysaccharides in mycelium cells and polysaccharides in fermentation broth. Pharmacological and drug efficacy tests have shown that besides antioxidant activity, the polysaccharides of Hericium erinaceus mycelium also have biological activities such as lowering blood sugar, lowering blood fat, anti-tumor, and immune regulation. In addition, Hericium erinaceus polysaccharide plays an important role in tre...
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