Microfluidic chip for separation and detection of whole blood sample, and detection method thereof

Abstract

The invention provides a microfluidic chip for separation and detection of a whole blood sample. The microfluidic chip comprises a chip body, which is provided with a sample flow channel, wherein thesample flow channel comprises a sample injection zone, a sedimentation zone, a mixing zone, a detection zone and a waste liquid zone, the sample injection zone, the sedimentation zone, the mixing zone, the detection zone and the waste liquid zone are sequentially connected, the sedimentation zone comprises a sample injection part and a sedimentation part, a ratio of the maximum width of the sedimentation part to the maximum width of the sample injection part is 2-10, the sedimentation part has a structure with the wide middle portion and both narrow edge portions, the front side walls and therear side walls of both end portions of the sedimentation part are respectively an inclined surface, the extending lines of the front side wall and the rear side wall intersect to form an included angle, and the front side wall and the rear side wall of the middle portion of the sedimentation part are parallel surfaces parallel to each other. According to the present invention, the microfluidic chip can combine the separation and the detection of the plasma in whole blood without the complicated whole blood sample pretreatment process, and can quickly and quantitatively detect the single or multiple proteins or other indicators in whole blood.

Description

technical field [0001] The invention relates to the technical field of fluid sample detection, in particular to a microfluidic chip for separation and detection of whole blood samples and a detection method thereof. Background technique [0002] Analysis of blood components and their content is the most basic item in modern medical testing. Whole blood is composed of liquid plasma and blood cells. Because blood cells or hemoglobin cause great interference to spectral analysis, it is usually necessary to separate plasma from blood samples and then use it for biochemical or immunodiagnostic analysis. At present, the most commonly used plasma separation methods clinically are centrifugation and filtration, but both methods have certain shortcomings. The centrifugal method has bulky equipment and complicated operation; the filtration method has low separation efficiency and the sample is easily polluted. [0003] At present, POCT (Point Of Care Testing, instant detection) tech...

AI Technical Summary

Problems solved by technology

The centrifugal method is bulky and complicated to operate; the filtration method has low separation efficiency and the sample is easily polluted

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