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Biological indicators

A biological indicator, biological technology, applied in the direction of biomass post-processing, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of incomplete inactivation of test organisms, unsuccessful sterilization cycles, etc.

Pending Publication Date: 2018-10-23
AMERICAN STERILIZER CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unsuccessful sterilization cycle resulting in incomplete inactivation of test organism (growth detected)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example

[0067] In the following examples, test organisms were prepared using a standard batch of spores of G. stearothermophilus suspended in an aqueous carbohydrate solution. In these examples, xylose and dextrose were used as carbohydrates. Also provided are comparative examples in which no carbohydrates were used. In each case, the aqueous suspension was placed in a vial and the suspension was allowed to dry. It's like Image 6 shown. The resulting biological indicators were tested for resistance. Tests showed that resistance was unexpectedly reduced when the biological indicator was derived from a carbohydrate solution compared to when no carbohydrate was used.

[0068] In these instances, use Figure 5A The Biological Indicator Evaluation Resistance Render (BIER) container depicted in BIER tests the resistance of biological indicators to steam sterilization. refer to Figure 5A , BIER container 300 includes a chamber 310, a door 320, and an internal shelf 330 for placing b...

example 1

[0074] The resistance of biological indicators to steam at 121 °C was evaluated in a BIER vessel. The first group of biological indicators was derived from a suspension of spores of Geobacillus stearothermophilus in water. A second set of biological indicators was derived from a suspension of spores in a 1.67M xylose solution. Compared with the first group of biological indicators without xylose solution (log reduction of 6 in about 20 minutes), when using the second group of biological indicators derived from xylose solution (log reduction in about 5 minutes When 6) is reduced, the resulting kill kinetics are significantly shorter. It's like figure 1 shown.

example 2

[0076] The test performed in Example 1 was repeated except that the test was performed at 132°C in the BIER vessel. This test showed a decrease in resistance from about 5 minutes (without xylose) to about 1.5 minutes (with xylose). The killing process was faster at 132°C than at 121°C. The addition of xylose further reduced the resistance. It's like figure 2 shown.

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Abstract

This invention relates to a biological indicator, comprising: a carrier inoculated with a test organism and an effective amount of a carbohydrate to reduce the resistance of the biological indicator to steam sterilization. The invention relates to a steam sterilization process using the biological indicator.

Description

technical field [0001] The present invention relates to biological indicators, and more particularly, to biological indicators that have been treated to reduce the resistance of the biological indicators to steam. Background technique [0002] Biological indicators, usually comprising a carrier and a test organism deposited on the carrier, are used to monitor the steam sterilization process. The biological indicators are placed in the sterilization chamber and passed through a steam sterilization cycle along with the load (eg, medical devices) to be sterilized. After the sterilization cycle, the biological indicators are exposed to the growth medium and incubated to determine if any test organisms survive. A successful sterilization cycle results in complete inactivation (no growth) of the test organism. Unsuccessful sterilization cycles resulted in incomplete inactivation (growth detected) of the test organism. Contents of the invention [0003] Biological indicators t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/22C12M1/12A61L2/07
CPCA61L2/07C12M37/06C12Q1/22A61L2/28C12M47/16
Inventor 威廉·A·伊拉瓦妮科尔·M·比恩菲利普·P·法兰西斯科维奇特丽夏·克里格
Owner AMERICAN STERILIZER CO
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