Direct infrared analysis of post-translational modification of proteins

A post-translational modification, protein technology, applied in the field of direct infrared analysis of protein post-translational modification, can solve the problem of no biomarkers

Pending Publication Date: 2018-11-09
UNIV GENT
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, to date, there are no clinically useful biomarkers for assessing carbamylation that are available in routine clinical laboratories

Method used

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  • Direct infrared analysis of post-translational modification of proteins
  • Direct infrared analysis of post-translational modification of proteins
  • Direct infrared analysis of post-translational modification of proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0092] Example: Infrared Spectroscopy of Diabetic and Control Nails

[0093] Near-infrared spectra were taken on the nail plates of 5 patients diagnosed with diabetes and 25 people in the control group, all belonging to the same race.

[0094] NIR spectrum at 4200cm -1 -5400cm -1 taken in the wavenumber range of and plotted at Figure 2a middle. It can be seen that the spectra between the two groups are clearly separated. To better quantify this spectral difference, for each measurement, the Figure 2b The standard normal variation (SNV) is listed in to analyze the spectra. A fairly good separation between the data points corresponding to the diabetic group on the one hand and the control group on the other hand can be observed.

[0095] To further improve the separation, the spectral range is reduced to 4200-4500cm -1 , and the obtained spectrum is as Figure 3a shown. Again, for each measurement, the spectra were analyzed by listing the SNVs in Fig. 3b. A further...

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Abstract

The present invention relates to a method for measuring post-translational modification of proteins in a subject. The method comprises recording of infrared radiation within a predetermined wavenumberrange, attenuated by an integument of said subject, wherein said integument is still attached to said subject, and comparing said attenuation of infrared radiation to a predetermined value for deriving information regarding post-translational modification of proteins in the integument. The invention also relates to a corresponding system as well as to a post-translational modified integument protein as marker for in vivo diagnosis of a disease, e.g. diabetes mellitus or renal insufficiency.

Description

technical field [0001] The present invention relates to measuring post-translational modifications of proteins in a subject, and more particularly to measuring post-translational modifications of proteins by using infrared spectroscopy, whereby post-translational modifications of proteins can be representative of diseases, like for example diabetes or renal insufficiency. Background technique [0002] Although diabetes prevalence is rising dramatically in sub-Saharan Africa, accurate diagnosis and monitoring of this metabolic disease by local health professionals remains problematic. Diabetes is diagnosed based on plasma glucose or hemoglobin A1c (HbA1c) concentrations according to revised criteria from the American Diabetes Association, the European Association for the Study of Diabetes, and the International Diabetes Federation. For plasma glucose concentrations, the diagnosis is based on: fasting plasma glucose concentration ≥126 mg / dL (7.0 mmol / L), random plasma glucose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/145
CPCA61B5/14532A61B5/0075A61B5/4842A61B5/4848A61B5/4866G01N21/35
Inventor J·德拉吉M·斯皮卡尔特T·蒙泰尼T·德比尔
Owner UNIV GENT
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