The utility model provides a kind of spotting positioning device for
cell culture multi-well plate, the spotting positioning device includes the lower layer bottom plate and upper layer spotting positioning rectangular frame of
parallel arrangement;The lower layer bottom plate is also equipped with and is used for supporting the support column of spotting positioning rectangular frame;The top surface of the lower layer bottom plate is formed with the multi-well plate limiting slot for placing multi-well plate;The upper layer spotting positioning rectangular frame is formed with rectangular work window;The spotting positioning device further includes replaceable spotting positioning strip, the spotting positioning strip is formed with several array spotting holes, the spotting positioning strip is erected on the upper layer spotting positioning rectangular frame so that spotting hole is located in rectangular work window;Positioning scale for identifying or assisting positioning spotting positioning strip position is equipped on the upper layer spotting positioning rectangular frame.The spotting positioning device of the application is simple and easy to operate, low in cost, and has high parallelism between multiple holes, suitable for general laboratory applications that do not require excessive samples and have low budgets.