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Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells

An observation device and a technology for immune cells, which can be used in methods for obtaining spatial resolution, measurement devices, animal cells, etc., and can solve problems that have not yet been disclosed.

Inactive Publication Date: 2018-11-09
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, an assay for specifically determining how many cancer cells one immune cell damages has not been published

Method used

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  • Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells
  • Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells
  • Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells

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Embodiment Construction

[0042] The preferred embodiments for implementing the present technology will be described below. It should be noted that the following embodiments describe typical embodiments of the present technology, and the scope of the present technology should not be interpreted narrowly thereby. The description will be made in the following order.

[0043] 1. Cell observation device

[0044] 1-1. Device configuration

[0045] 1-2. Examples of cell observation and analysis

[0046] 1-3. Data analysis

[0047] 2. Methods for assessing the level of immune cell activity

[0048] 2-1. Evaluation by cytotoxicity assay

[0049] 2-2. Evaluation by secretion release measurement

[0050] 2-3. Through antibody-dependent cytotoxicity assessment

[0051] 2-4. Application example 1

[0052] 2-5. Application example 2

[0053] 3. Methods for controlling the quality of immune cells

[0054]

[0055] 1-1. Device configuration

[0056] figure 1 An example of a cell observation device according to the present technology...

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Abstract

The purpose of the present invention is to provide: a method for quantitatively evaluating the activity of individual cells; and a device used therefor. This cell observation device is provided with acell introduction part, a cell arrangement part, an observation part, and an analysis part, wherein: the cell introduction part introduces one or more cells into the cell arrangement part; the cell arrangement part arranges the introduced one or more cells; the observation part makes an observation about temporal events resulting from cell contact in the cell arrangement part; and the analysis part analyzes the temporal events resulting from the cell contact.

Description

Technical field [0001] The present invention relates to a cell observation device, a method for evaluating the activity level of immune cells, and a method for controlling the quality of immune cells. Background technique [0002] In the past, in the determination of cytotoxicity related to a variety of cells, a technique using radioisotopes (Cr51 release measurement) was used. However, this method is limited in operation and requires complicated operations. In addition, Cr51 must be introduced into the cell to be damaged at one time, and its degree varies according to the type of cell, which is the cause of the variable measurement accuracy. [0003] In the lactate dehydrogenase (LDH) assay, which is a technique that does not use radioisotopes, the damage to the cell can be measured by measuring the lactate dehydrogenase (LDH) released from the cell. However, according to this technique, it is impossible to distinguish which target cells and effector cells are associated with me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/02C12N5/078C12N5/09
CPCC12M1/34C12Q1/02G01N33/5011G01N33/5047A61K39/4632A61K39/4644A61K39/4611C12N5/0634G01N33/5008C12M23/12C12M23/58G01N21/01G01N21/47G01N2021/0106G01N2021/0181G01N2021/0193G01N2021/178
Inventor 中村友彦马尔科雷勒·布兰
Owner SONY CORP
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