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A method for promoting reduction of chromium by dissimilating iron reducing bacteria by taking pyrite as electron shuttle carrier

A technology of shuttle carrier and dissimilatory iron, applied in the field of biological treatment of pollutants, can solve problems such as inability to exert effects, and achieve the effects of easy promotion, high efficiency and low cost

Inactive Publication Date: 2018-11-13
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pyrite has a wide range of sources, and its Fe(II) and S- ions have a certain reduction ability to Cr(VI), but it is passivated under neutral conditions and cannot exert its effect.

Method used

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  • A method for promoting reduction of chromium by dissimilating iron reducing bacteria by taking pyrite as electron shuttle carrier
  • A method for promoting reduction of chromium by dissimilating iron reducing bacteria by taking pyrite as electron shuttle carrier
  • A method for promoting reduction of chromium by dissimilating iron reducing bacteria by taking pyrite as electron shuttle carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method described in this example mainly includes the following steps:

[0033] (1) Insert Shewanella oneidensis MR-1 into LB liquid medium for enrichment culture, the initial pH of the culture is 7, the temperature is 30°C, and the shaker speed is 170rpm. After culturing for 12 hours to the logarithmic growth phase, the collected culture solution was collected by centrifugation at 6000 rpm, 8 minutes, and 20°C. Wash the collected thalline twice with bicarbonate buffer, then suspend the thalline in the buffer to obtain a bacterial suspension;

[0034] (2) Add the bacterial suspension in step (1) to 100mL basal medium in the anaerobic operation box, and the final concentration of the bacterial strains in the system is 4×108 / mL. And add 20mM sodium lactate, 0.01g pyrite to the system in the anaerobic operation box, and add different concentrations of Cr(Ⅵ) solutions (15.6, 25, 36, 50 mg / L) respectively. At the same time, set blank control group: basal medium + Cr(Ⅵ) ...

Embodiment 2

[0038] The method described in this example mainly includes the following steps:

[0039] (1) Insert Shewanella oneidensis MR-1 into LB liquid medium for enrichment culture, the initial pH of the culture is 7, the temperature is 30°C, and the shaker speed is 170rpm. After culturing for 12 hours to the logarithmic growth phase, the collected culture fluid was collected by centrifugation at 6000 rpm, 8 minutes, and 20°C. Wash the collected bacteria twice with bicarbonate buffer, and then suspend the bacteria in the buffer to obtain a bacterial suspension;

[0040](2) Add the bacterial suspension in step (1) to 100mL basal medium in the anaerobic operation box, and the final concentration of the bacterial strains in the system is 4×108 / mL. And in the anaerobic operation box, 20mM sodium lactate, 25 mg / L Cr(VI) solution, and different concentrations of pyrite (0.1g / L, 1g / L) were added to the system. At the same time, set blank control group: basal medium + Cr(Ⅵ) solution; biolog...

Embodiment 3

[0044] The method described in this example mainly includes the following steps:

[0045] (1) Insert Shewanella oneidensis MR-1 into LB liquid medium for enrichment culture, the initial culture pH is 7, the temperature is 30°C, and the shaker speed is 170rpm. After culturing for 12 hours to the logarithmic growth phase, the collected culture fluid was collected by centrifugation at 6000 rpm, 8 minutes, and 20°C. The collected bacterial cells were washed twice with bicarbonate buffer solution, and then the bacterial cells were suspended in the buffer solution to obtain a bacterial suspension. Air-dry the chromium residue soil with a chromium content of 530 mg / kg, grind it finely, and pass a 0.25 mm soil sieve;

[0046] (2) Add the bacterial suspension in step (1) to 100mL basal medium in the anaerobic operation box, and the final concentration of the bacterial strains in the system is 4×108 / mL. And add 20mM sodium lactate, 6g of chromium slag soil and 0.01g of pyrite to the s...

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Abstract

The invention discloses a method for promoting the reduction of chromium by dissimilating iron reducing bacteria by taking pyrite as an electron shuttle carrier and belongs to the technical field of pollutant biological treatment. The method comprises the following steps: adding 0.1-1.5 g / L of pyrite in a basic ion culture medium containing Cr(VI), transferring into a serum bottle, adding the dissimilating iron reducing bacteria in the serum bottle and then putting in a constant-temperature shaker of 160-220 rpm and 25-35 DEG C for dark reaction for 40-60 hours. The pyrite is passivated underneutral conditions and basically has no reduction treatment effect on the Cr(VI), the separate treatment effect of the dissimilating iron reducing bacteria on the Cr(VI) is not good, and when the dissimilating iron reducing bacteria is combined with the pyrite to reduce the Cr(VI), the reduction rate of the Cr(VI) is significantly improved and is 2-5 times higher than that of the Cr(VI) reduced bythe separate use of the dissimilating iron reducing bacteria. The pyrite and the dissimilating iron reducing bacteria are widely available, and the method is low in chromium pollution treatment cost,high in efficiency and easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of pollutant biological treatment, and in particular relates to a method in which pyrite is used as an electron shuttle carrier to promote the reduction of chromium by dissimilatory iron-reducing bacteria. Background technique [0002] Chromium is widely used in many industrial productions, such as stainless steel, leather, and electroplating, and a large amount of chromium-containing wastewater is produced. If it is not treated properly, it will cause huge pollution to the environment and pose a threat to human health. The common valence states of chromium are Cr(VI) and Cr(III). Cr(Ⅵ) has good solubility in water and strong toxicity, and is listed as an important pollutant by the US Environmental Protection Agency. However, Cr(Ⅲ) can form precipitates, has good thermodynamic properties and low toxicity, so the common way to deal with chromium pollution is to convert Cr(Ⅵ) into Cr(Ⅲ). [0003] Dissimilato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
CPCC12P3/00
Inventor 朱建裕章可甘敏王旭朱敬磊刘梦飞
Owner CENT SOUTH UNIV
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