Electrochemical biological sensor for detecting alpha fetal protein and preparation method thereof

A technology of biosensors and alpha-fetoprotein, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high price, complicated operation, and inability to detect in real time, and achieve good specificity, fast and accurate recognition

Inactive Publication Date: 2018-11-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the reported technologies still have many problems such as complicated operation, high price, and incapable of real-time detection.

Method used

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  • Electrochemical biological sensor for detecting alpha fetal protein and preparation method thereof
  • Electrochemical biological sensor for detecting alpha fetal protein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An electrochemical biosensor for detecting alpha-fetoprotein, the preparation method of the sensor comprises the steps of:

[0024] (1) Preparation of Au@Ag core-shell nanoparticles: Take 20 mL of gold nanoparticles with a particle size of 17.8±1.8 nm, centrifuge at 8000 r / min for 10 minutes, concentrate 10 times, and redissolve in 2 mL of ultrapure water. Get 4mL, the phosphate buffer solution of pH=7.4 and the polyvinylpyrrolidone solution that 2mL concentration are 1% (wt%) mix homogeneously, then add 2mL gold nanoparticle solution and 1mL concentration be the ascorbic acid solution of 0.1mol / L thereafter, Finally, 6.4 mL of silver nitrate solution with a concentration of 3 mmol / L was added, and Au@Ag core-shell nanoparticles were obtained after 3 hours of reaction under uniform shaking conditions, which were centrifuged and washed to a volume of 1 mL for later use. The transmission electron microscope image of the obtained Au@Ag core-shell nanoparticles is shown in ...

Embodiment 2

[0030] With reference to the steps of Example 1, prepare an electrochemical biosensor for detecting alpha-fetoprotein, and apply it to clinical trials:

[0031] Firstly, centrifuge 2mL of the blood of the liver cancer patient at 400g for 5 minutes to obtain 1mL of serum, which is diluted to 2mL with PBS solution for later use; then the accurate concentration of AFP is measured by a chemiluminescence immunoassay analyzer, and then diluted to 45pg / mL. Take 5 μL of the prepared diluted serum sample and drop it on the electrode modified by Au@Ag core-shell nanoparticles. Refer to step (4) for electrochemical detection. Three groups were measured in parallel, and the content of alpha-fetoprotein was measured to be 44.2pg / mL, the error is 1.8%, and the detection result is relatively accurate.

Embodiment 3

[0033] With reference to the steps of Example 1, prepare an electrochemical biosensor for detecting alpha-fetoprotein, and apply it to clinical trials:

[0034] Firstly, 2 mL of blood from patients with liver cancer was centrifuged at 400 g for 5 minutes to obtain 1 mL of serum, which was diluted to 2 mL with PBS solution for use. Then, the accurate concentration of AFP was measured by a chemiluminescence immunoassay analyzer, which was diluted to 75pg / mL, and 5 μL of the prepared diluted serum sample was dropped onto the Au@Ag core-shell nanoparticle-modified electrode, referring to the steps ( 4) Electrochemical detection was carried out, 3 groups were measured in parallel, and the content of alpha-fetoprotein was measured to be 72.8pg / mL, with an error of 2.9%, and the detection result was relatively accurate.

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Abstract

The invention discloses an electrochemical biological sensor for detecting alpha fetal protein. A preparation method of the electrochemical biological sensor comprises the following steps: (1) preparing Au@Ag core-shell nanoparticles; (2) modifying the Au@Ag core-shell nanoparticles by the alpha fetal protein; (3) preparing a sulfanilic acid modified bare glassy carbon electrode; (4) preparing theelectrochemical biological sensor. The electrochemical biological sensor for detecting the alpha fetal protein is prepared for the first time by utilizing the Au@Ag core-shell nanoparticles having adjustable electrochemical activity, and the electrochemical biological sensor is convenient to operate and has very high sensitivity.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor based on an Au@Ag core-shell nanoparticle modified electrode for detecting alpha-fetoprotein. Background technique [0002] Alpha-fetoprotein is a major serum protein in early embryonic development and is produced by liver cells in adults with extremely low levels. The level of alpha-fetoprotein in serum is related to liver cancer, embryonal tumors and some extrahepatic tumors. The expression of alpha-fetoprotein in tumor cells is characterized by high efficiency and specificity, and its content is closely related to the severity of the disease. Therefore, the content of alpha-fetoprotein in serum can not only be used as one of the early diagnosis indicators of primary liver cancer and teratoma, but also can be used as an indicator for curative effect observation and prognosis judgment. [0003] At present, the detection methods of alpha-fetoprotein mainly include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/3275G01N27/3277G01N27/48
Inventor 赵媛杨亚鑫杨璇崔林艳
Owner JIANGNAN UNIV
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