A kind of preparation method of carbon dot drug loading system

A technology of carbon dots and systems, applied in the field of biological sciences, can solve the problems of reduced release rate, low bioavailability, toxic and side effects of ferric citrate taste, etc., to achieve the effects of reducing toxic and side effects, simple preparation method, and improving drug efficacy

Active Publication Date: 2021-08-17
HEBEI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of a carbon dot drug-carrying system. The obtained CDs-DOX has slow-release properties in buffer solutions with different pH values, and the release rate decreases successively, overcoming the disadvantage of doxorubicin hydrochloride. Anticancer drugs have toxic and side effects and iron citrate has the disadvantages of poor taste and low bioavailability

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  • A kind of preparation method of carbon dot drug loading system
  • A kind of preparation method of carbon dot drug loading system
  • A kind of preparation method of carbon dot drug loading system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 A method of preparing carbon dots (CDs) from peony pollen

[0026] Put the picked peony petals into an electric blast drying oven, dry them at 80°C, grind the dried petals into pollen, and seal them for storage. according to m 牡丹粉 :m 尿素 =1:3 Weigh peony powder and urea and put them into a beaker. Add 10mL of water, stir with a magnetic stirrer, and transfer to a polytetrafluoroethylene liner after uniformity. Then put the reaction kettle into an electric blast drying oven and heat at 200° C. for 10 hours to obtain a brown-black solution. Filter to remove large particle impurities, centrifuge at 4000rmp / min for 10 minutes, take the supernatant after standing for several hours, and dialyze for 6 hours to remove excess modification reagent urea to obtain carbon dots. Take a certain volume of CDs solution and dry it in an oven at 80°C, weigh the mass of the obtained solid carbon dots, and calculate the mass concentration of the carbon dot solution.

Embodiment 2

[0027] Example 2 A method for preparing a carbon dot-doxorubicin (CDs-DOX) drug-loading system from peony pollen (drug loading 37.2%)

[0028] (1) Preparation of CDs-Doxorubicin (CDs-DOX) drug-loading system

[0029] The carbon dots (CDs) used were obtained by the method in Example 1. According to mass ratio m CDs :m DOX =1:2 to prepare the solution, place it on a shaker to react in the dark for 12 hours, and dialyze to remove free DOX to obtain CDs-DOX.

[0030] (2) Calculation of drug loading in CDs-Doxorubicin (CDs-DOX) drug loading system

[0031] Draw the standard curves of CDs and DOX at wavelengths of 330nm and 488nm respectively, dilute the prepared CDs-DOX solution 10 times, measure its absorbance at 330nm and 488nm with a UV spectrophotometer, calculate the concentration according to the standard curve, according to the following Formula to calculate drug loading:

[0032]

Embodiment 3

[0033]Example 3 A method of preparing carbon dots-ferric citrate (CDs-FAC) drug-loading system from peony pollen (drug loading 54.0%)

[0034] (1) Preparation of CDs-ferric citrate (CDs-FAC) drug-loading system

[0035] The carbon dots (CDs) used were obtained by the method in Example 1. According to mass ratio m CDs :m FAC =1:1 for solution preparation, reacted on a shaker in the dark for 12 hours, dialyzed to remove free FAC, and prepared CDs-FAC.

[0036] (2) Calculation of drug loading in CDs-Ferric Citrate (CDs-FAC) drug loading system

[0037] Draw the standard curve of FAC at a wavelength of 260nm, take the release medium outside the dialysis bag after the above-mentioned dialysis, measure its absorbance at 260nm with a UV spectrophotometer, calculate the concentration according to the standard curve, and calculate the drug loading according to the following formula:

[0038]

[0039] Explanation: the abbreviation formula of the chemical substance used among the ...

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Abstract

The invention discloses a preparation method of a carbon dot drug loading system. Using peony pollen as raw material and urea as a modification reagent, carbon dots with uniform particle size were prepared by hydrothermal method, and then the carbon dots were respectively reacted with doxorubicin hydrochloride (DOX) and ferric citrate (FAC) in a dark coupling reaction. , to obtain CDs‑DOX and CDs‑FAC. The CDs-DOX prepared by the present invention has high drug loading, sustained release, and has the characteristics of targeted release of cancer cells; the prepared CDs-FAC has high drug loading, slow release, and gastric juice release is slower than intestinal juice Release, CDs‑FAC can reduce the stimulating effect of FAC on the stomach, facilitate absorption in the small intestine, and is suitable for oral administration. The preparation method is simple, the operation is convenient, and the reaction is easy to control; the obtained product is beneficial to improving the administration effect of simple DOX or FAC, reducing its toxic and side effects, and can be used for treating diseases related to iron disorder.

Description

technical field [0001] The invention relates to a method for preparing a drug-loading system, in particular to a method for preparing drugs related to iron disorder diseases carried by carbon dots, and belongs to the technical field of biological sciences. Background technique [0002] Iron is an essential trace element for the human body, and iron deficiency or iron overload can lead to disease states in the human body. Iron overload can lead to peroxidative damage and increase the risk of cancer. Studies have shown that iron overload is related to the occurrence and development of various cancers. Cancer is a major killer that threatens human health and quality of life today, and it is also one of the main causes of death. Current clinical treatment methods include surgical resection, chemotherapy, radiotherapy, and integrated traditional Chinese and Western medicine. In the chemotherapy process of traditional cancer treatment, doxorubicin hydrochloride is a commonly use...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K47/52A61K31/704A61K31/295A61P35/00A61P3/02C01B32/15
CPCA61K31/704A61K33/26A61K47/52A61P3/02A61P35/00C01B32/15
Inventor耿丽娜张泽帝段相林常彦忠
OwnerHEBEI NORMAL UNIV