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A kind of fermented carbonized Pu'er tea granule and its preparation method and application

A Pu-erh tea and granule technology, which is applied in the application, tea treatment before extraction, tobacco, etc., can solve the problems of insignificant adsorption effect of harmful substances in smoke, poor coordination of cigarette aroma, and inability to add aroma to cigarettes, etc., to achieve good business Application value, convenient industrial production, low cost effect

Active Publication Date: 2021-10-22
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the disadvantages of the existing plant particles, such as insignificant adsorption effect on smoke harmful substances, inability to directly add aroma to cigarettes, or poor coordination with cigarette aroma, etc., the present invention provides a fermented carbonized Pu-erh tea particle and its preparation method and application, It modifies the plant raw materials by using the fermenting and carbonization process of the tobacco soil Dailella L4-6 to provide a kind of cigarette that can not only enhance the effect of reducing harm and reducing tar of cigarettes, reduce the irritation of cigarette smoke, but also enhance the effect of cigarette aroma and Improving the quality of cigarette smoking

Method used

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  • A kind of fermented carbonized Pu'er tea granule and its preparation method and application
  • A kind of fermented carbonized Pu'er tea granule and its preparation method and application
  • A kind of fermented carbonized Pu'er tea granule and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Isolation, cultivation and identification of Tobacco Solanum Daiia L4-6

[0040]1.1 Isolation of Tobacco Soil Daeella L4-6

[0041] Tobacco soil samples were taken from the tobacco planting base in Kunming, Yunnan, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 90mL of sterile water, shake on a shaker at 30°C and 180rpm for 30min; dilute the concentration to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Take 0.2mL of the five concentrations of bacterial solution and spread it on the LB plate medium, set up three parallel experiments for each concentration of the bacterial solution, cultivate it at 30°C for 48 hours, and pick it from the plate of each soil sample For different single colonies, after purification on LB plate medium, 20% of the bacterial volume was added to glycerol, and stored in a -80°C refrigerator for later use.

[0042] The isolated microorganisms were respectively inoculated in LB liq...

Embodiment 2

[0058] 1. Cultivation of Tobacco Soil D. L4-6

[0059] (1) Test tube slant culture

[0060] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate it with the strain L4-6 of Agrobacterium nicotianae, and cultivate it at 28°C for 1 week to obtain the strains in the test tube;

[0061] (2) Seed cultivation

[0062] Adopt seed medium, the formula of seed medium is: dextrin 120g, soybean powder 40g, yeast extract 2g, tryptophan 0.5g, β-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, pick pa...

Embodiment 3

[0073] 1. The culture method of Tobacco Soil Dainella L4-6 is the same as in Example 2.

[0074] 2. Fermentation of Pu-erh tea

[0075] Weigh 600g of Pu-erh tea that has been pre-balanced to 12% moisture, spray 120mL of the inoculum of Agrobacterium nicotiana L4-6, put the treated Pu-erh tea in a constant temperature and humidity box at 22°C and 60% for fermentation for 72 hours.

[0076] 3. Carbonization of fermented Pu-erh tea

[0077] Put the fermented Pu’er tea into the carbonization furnace, gradually raise the temperature of the furnace to 80°C, and keep it for 30 minutes. After the Pu’er tea fermented sample is dried, quickly raise the temperature to 140°C, and keep it for 2.5 hours. After checking the degree of carbonization, turn off the heat source. Take out the fermented carbonized Pu-erh tea sample for later use.

[0078] 4. Preparation of fermented carbonized Pu-erh tea granules

[0079] Take fermented carbonized Pu'er tea, detect its water content, make up the...

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Abstract

The invention provides a fermented carbonized Pu'er tea granule and its preparation method and application, which are prepared by the method comprising the following steps: (1) preparation of tobacco soil D. Bacteria L4‑6 bacterial agent ferments Puer tea to obtain fermented Puer tea; (3) carbonizes fermented Puer tea to obtain fermented carbonized Puer tea; (4) places fermented carbonized Puer tea powder on a fluidized bed drying, spraying binder, binding powder to form granules, and continuing to dry to obtain fermented carbonized Pu'er tea granules. The granules are fermented and applied to cigarettes to produce a special aroma that harmonizes with cigarette smoke, which not only enriches the aroma of cigarettes, but also has pharmacological effects such as relieving throat discomfort, promoting body fluid and quenching thirst; after carbonization treatment, it can significantly increase The internal adsorption specific surface area of ​​Pu'er tea strengthens the adsorption of particles to harmful substances in cigarette smoke, and at the same time endows plant particles with a special burnt aroma.

Description

technical field [0001] The invention belongs to the technical field of cigarette materials, and in particular relates to a fermented carbonized Pu'er tea granule and a preparation method and application thereof. Background technique [0002] Low coking has become an inevitable trend in the development of the tobacco industry. Low coking brings about a reduction in the taste of cigarettes, and insufficient aroma has gradually become a prominent problem in the development of tobacco. How to overcome the key technologies that affect the aroma and aroma of tobacco leaves has become the core of improving the competitiveness of cigarette production. Adding tobacco flavors and fragrances to shredded cigarettes is a conventional and necessary technical means to add aroma, but the space for improving the quality of cigarettes is relatively limited. It has become a new development trend to improve the aroma of cigarettes and reduce the irritation of cigarette smoke. Among them, addi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23F3/10A23F3/06A24B15/20
CPCA23F3/06A23F3/10A24B15/20
Inventor 刘秀明段焰青李文均杨登强王凯张翼鹏高莉
Owner CHINA TOBACCO YUNNAN IND
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