63results about How to "Good business value" patented technology

The invention discloses a real-time guidance method for bus taking navigation and belongs to the technical field of intelligent traffic. The method comprises the following steps: determining an origin, a destination and departing time; carrying out path planning according to the coordinates of the origin and the destination, and obtaining the time cost of each road section in the path planning according to the departing time and a path planning result in combination with dynamic traffic data; correcting the time cost of each road section according to the actual departing time and the current position of a user and utilizing characters, voice and/or graphical representations to carry out real-time guidance at each road section. On the basis of providing the character and map demonstration of the path planning result, the real-time guidance method can further provide the progress bar of journey stages and expected time for arriving at each stage point, and can also provide a whole-journey real-time guidance function. The real-time guidance method has the advantages of simple and visual journey demonstration, timely and accurate prompt, and safety and convenience in use. The use convenience of passengers in navigation is greatly improved, and the occurrence of accidents is reduced when navigation equipment is used during motion.

The invention discloses a Hyphomicrobium sp. strain and a preparation method for pyrroloquinoline quinone. The Hyphomicrobium sp. strain 1112-NTG-2318 capable of realizing high yield of pyrroloquinoline quinine is preserved in China General Microbiological Culture Collection Center with an accession number of CGMCC No. 10709. The preparation method for pyrroloquinoline quinine comprises the following steps: fermenting the Hyphomicrobium sp. strain CGMCC No. 10709 in a fermentation medium and acquiring pyrroloquinoline quinine from fermentation broth. The fermentation titer of the preparation method in a tank with a volume of 80 m<3> reaches 1783 mg/L, so the preparation method is favorable for industrial production of pyrroloquinoline quinine.

The invention discloses a double-promoter multi-copy recombinant pichia pastoris engineering strain for highly producing endo-inulinase INU2. The strain is named pichia pastoris GS115-A3-G2, and is preserved in the general microbiology center of the China Committee for Culture Collection of Microorganisms on April 16th, 2014, and the preservation number is CGMCC NO:9049. According to the strain disclosed by the invention, the endo-inulinase gene INU2 multi-copy is integrated into pichia pastoris GS115 genome by two plasmids pPIC9K and pGAPZaA, and the double-promoter multi-copy recombinant pichia pastoris engineering strain for efficiently expressing the endo-inulinase is obtained in a screening manner. The experiment proves that the inulinase enzyme activity can be up to 3006U/ml in 3L high-density fermentation, simply purified crude enzyme can be directly applied to hydrolysis of inulin, so as to generate fructo-oligose, the conversion efficiency is high, production of the fructo-oligose by using inulin enzymatic hydrolysis in industry is achieved, and the double-promoter multi-copy recombinant pichia pastoris engineering strain has great practical value.

The invention provides a method for manufacturing a magnesium boride superconductive belt material. The method comprises the following steps: A, uniformly distributing amorphous boron on a conductive substrate by adopting a coating method to form an amorphous boron layer with thickness of between 0.1 and 2.0mm; B, covering a magnesium or magnesium alloy flake with thickness of between 0.1 and 2.0mm on the boron layer, and rolling to form a magnesium-boron-substrate combination; C, putting the combination in the step B in a thermal treatment furnace, under the protection of argon atmosphere, heating to the temperature of between 550 and 800 DEG C, preserving the temperature for 0.1 to 3 hours, and cooling; D, coating metal, metallic oxide, silicon carbide or diamond like carbon film on the combination which is treated in the step C to serve as a protective layer to form a composite body; and E, cutting the composite body in the step D into long belts. The MgB2 superconductive belt prepared by the method has the advantages of good compactness and grain connection, high critical current density, uniform current density distribution and high fatigue life, and is suitable for commercial application. The method is simple, and is suitable for industrial production.

The invention belongs to the field of hard clay sampling, and particularly relates to a hard clay undisturbed three-axis sample preparation device and a using method thereof. The device comprises a support, wherein a base is arranged at the bottom of the support; a bottom plate is arranged on the base; a lower fixing disc is arranged in the middle of the bottom plate; an upper fixing disc is arranged at a position above the lower fixing disc; the upper fixing disc is connected with a guiding rod; the guiding rod penetrates through a tool cap; the tool cap is connected with a hollow spiral tool; the tool cap is in threaded connection with a feeding guide track; the feeding guide track is a threaded rod, penetrates through the top of the support and then is connected with an output end of a stepping motor; and the stepping motor is positioned on the top of the device and is controlled by a controller. The hard clay undisturbed three-axis sample preparation device is skillful in structural design; the size and perpendicularity of a soil sample can be controlled accurately; success rate of soil sample preparation is high; sample preparation time is saved; the sample preparation efficiency is greatly improved; and the business application value is high.

The invention discloses a PNO ligand containing planar chiral ferrocene and application thereof. The PNO ligand containing planar chiral ferrocene is a planar chiral ferrocene-containing and phenol-containing PNO ligand as shown in a general formula (I) or (II) which is described in the specification, or a planar chiral ferrocene-containing and aryl-phosphoric-acid-containingPNO ligand containing as shown in a general formula (III) or (IV) which is described in the specification, or a planar chiral ferrocene-containing and carbon-chiral-phenol-containingPNO ligand as shown in a general formula (V) or (VI) which is described in the specification. The invention provides tridentate PNO ligands and processes for their complexation with transition metal salts or transition metal complexes; the introduction of salicylaldehyde and derivatives thereof, which are simple and easy to obtain, enables the ligands to have a bifunctionalization effect, and -OH in a formed catalyst has stronger acidity and is beneficial to combination with N/O in polar double bonds. Therefore, due to the bifunctionalization effect of the catalyst, the interaction between the catalyst and a substrate can be greatly improved, so a reaction can obtain higher catalytic activity and stereoselectivity.

The invention provides a microcapsule fire extinguishing agent and a preparation method thereof. The microcapsule fire extinguishing agent comprises a flame-retardant shell composed of a low-melting-point copolymer and a cooling inner core containing a cooling material, wherein the flame-retardant shell coats the cooling inner core to form microcapsule particles with a core-shell structure, the low-melting-point copolymer is formed by copolymerizing a low-melting-point high-molecular polymer monomer and a flame retardant, the flame-retardant shell melts and explodes automatically when the external environment reaches the preset starting temperature, and the cooling inner core in the flame-retardant shell is released. The microcapsule fire extinguishing agent provided by the invention adopts an in-situ polymerization preparation method, and the microcapsule fire extinguishing agent with different starting temperatures is prepared by regulating and controlling the reaction parameters such as the variety of low-melting-point high-molecular polymer monomers, the mass ratio of the low-melting-point high-molecular polymer monomers to the cooling material, the copolymerization reaction time and pressure and the like, so that the microcapsule fire extinguishing agent is suitable for different scene requirements. The microcapsule fire extinguishing agent provided by the invention has targeting property, also has dual effects of cooling and fire extinguishing, and has huge application value.

The invention discloses an Alpha-galactosidase and a coding gene thereof. The alpha-galactosidase is a protein shown in a) or b) as follows: a) a protein consisting of amino acid residue from the 21-438th position of end N in sequence 1 in a sequence table; b) a protein consisting of the amino acid sequence shown in the sequence 1 in the sequence table; or c) a protein that derives from a) or b),has the activity of Alpha-galactosidase and is obtained from the protein limited by a) or b) through substitution and /or loss and/or addition of one or a plurality of amino acid residues. Applied towider industrial production, the enzyme provides experimental materials for the construction of further industrial high-yield engineering strain and also provides comparative sequences and property research reference for richening the diversity research of the source of Alpha-galactosidase. Therefore the enzyme and the coding gene have extremely favorable commercial application value and potential.

The invention discloses a locked nucleic acid probe fluorescent quantitative PCR detection composition, a detection method and a detection kit for African swine fever virus wild strain. The detectioncomposition is designed by utilizing characteristics of the locked nucleic acid, has strong recognition capability and affinity to DNA, can effectively improve detection efficiency of the African swine fever virus wild strain, and reduces probability of missed detection. Compared with an existing detection method, the detection method has advantages of strong specificity, high sensitivity and thelike, and is 10 times higher than that of a conventional TaqMan probe fluorescence quantitative PCR method. The detection composition, detection method and detection kit can be used to distinguish between African swine fever virus wild strains and MG360-505R gene-deleted vaccine strains, and are particularly suitable for scientific research and clinical applications, and have good commercial application value.

The invention relates to the field of cigarette packaging, in particular to a cigarette pile film wrapping method. The cigarette pile wrapping method comprises the following steps that firstly, a cigarette pile is subjected to film wrapping in a surrounding mode in the conveying direction to form a first sleeve film, and then the first sleeve film is conveyed to a supporting platen through an input conveying belt; secondly, a surrounding film wrapping device is arranged at the rear end of the supporting platen, an output conveying belt is arranged behind the surrounding film wrapping device, a cigarette pile pushing mechanism used for pushing the cigarette pile is arranged at the position of the input conveying belt, when the cigarette pile reaches the supporting platen, the cigarette pile is pushed through the cigarette pile pushing mechanism, and winding film wrapping of the cigarette pile are achieved through the surrounding film wrapping device until the cigarette pile reaches the output conveying belt; and thirdly, after the cigarette pile is subjected to the traction force of the output conveying belt, the cigarette pile pushing mechanism returns to the initial position to prepare for next-time pushing. According to the cigarette pile film wrapping method, the technical problems that the efficiency is not high and the stability is not good in the film wrapping process of existing cigarette piles are solved.

The invention discloses a method for purifying echinocandin B parent nucleus. The method comprises the following steps: 1) performing solid-liquid separation on a conversion solution; 2) concentrating, and removing the moisture from the liquid obtained in the step 1); 3) crystallizing; and 4) separating crystals. The crystallizing method used in the method disclosed by the invention is simple andconvenient to operate, time-saving, low in cost and suitable for continuous production on various scales; the product obtained by crystallization is high in purity; and the adopted non-polar solvent is easily recycled, and the cost is reduced.

The invention discloses an aluminum alloy material, an aluminum alloy wire and a preparation method of the aluminum alloy wire, and relates to the field of aluminum alloys. The aluminum alloy material comprises, by mass, 0.1%-0.25% of Fe, 0.01%-0.05% of Si, 0.02%-0.3% of Zr, 0.1%-1% of M, 0.02%-0.3% of Y, Mn, V, Ti, Cr and the balance is Al, wherein the total content of Mn, V, Ti and Cr is controlled to be smaller than or equal to 0.01%, the mass ratio of Fe to Si is 2-8, and M is composed of La and Ce. According to the aluminum alloy material, specific components are selected according to a specific ratio, on the basis that the cost is not obviously increased, the heat resistance and the mechanical property of the aluminum alloy material are effectively considered and enhanced, the conductivity is improved, and the aluminum alloy material has excellent commercial application value.

The invention relates to a kit for synchronously detecting African swine fever virus and porcine pseudorabies virus. The kit is a multiplex fluorescent quantitative PCR kit, and comprises primer pairs for amplifying an African swine fever virus CD2v gene, an African swine fever virus P72 gene and a porcine pseudorabies virus EPO gene, and corresponding fluorescent probes, wherein the nucleotide sequences of the primer pair for amplifying the CD2v gene and the corresponding fluorescent probe thereof, the primer pair for amplifying the P72 gene and the corresponding fluorescent probe thereof, and the primer pair for amplifying the EPO and the corresponding fluorescent probe thereof are as shown in SEQ ID NO: 1-9 correspondingly.

The invention provides a CHO-DHFR <+> cell strain and an application thereof. The preservation number of the cell strain is CCTCC (China Center For Type Culture Collection) NO: C2019264. The CHO-DHFR<+> cell strain can be suspension-cultured at high density without serum, normal growth and production of cells can be maintained only by adding a small amount of glutamine into a culture system in the culture process, accumulation of toxic waste NH4 <+> generated by glutamine metabolism is reduced, the cell doubling level is high, and the cell viability is high. A pcDNA3.1-beta recombinant plasmid vector containing an IL-10 gene sequence is transfected into the CHO-DHFR <+> cell strain to obtain an engineering cell strain for expressing recombinant IL-10, which is cultured in a CD OptiCHO medium containing G418, and efficiently express the recombinant IL-10. The transient expression quantity of the recombinant IL-10 protein is greater than 0.96 mg/L, so that the CHO-DHFR <+> cell strain has relatively good commercial application value.