Wide-spectrum bacillus for inhibiting aquatic product pathogenic vibrio and compound bacterial preparation thereof

The technology of Bacillus subtilis and Bacillus subtilis is applied in the field of Bacillus subtilis BSXE-1601, the preparation of compound bacteria preparations, and the field of compound bacteria preparations, which can solve the enhancement of drug resistance of diseased strains, reduce the immunity of cultured organisms, and the damage of cultured organisms. and other problems, to achieve the effect of promoting growth, improving aquaculture water environment, and promoting absorption and utilization.

Active Publication Date: 2018-11-23
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For many years, the prevention and treatment of Vibrio diseases has mainly been treated with chemical drugs such as antibiotics and disinfectants. The extensive use of antibiotics will lead to the emergence of a large number of drug-resistant strains through natural selection, making

Method used

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  • Wide-spectrum bacillus for inhibiting aquatic product pathogenic vibrio and compound bacterial preparation thereof
  • Wide-spectrum bacillus for inhibiting aquatic product pathogenic vibrio and compound bacterial preparation thereof
  • Wide-spectrum bacillus for inhibiting aquatic product pathogenic vibrio and compound bacterial preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] Example 1 Screening and isolation of Bacillus subtilis BSXE-1601

[0046] Collect the water body, bottom mud and intestinal feces of the prawns from the prawn farms, use the nutrient broth medium for bacterial enrichment, and separate the pure culture by streaking on the plate.

[0047] The isolated single strains were inoculated on nutrient agar plates coated with Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio vulnificus and Vibrio harveyi, by observing whether there is a bacteriostasis zone, and finally screening a pair of These 4 kinds of Vibrio strains with good antibacterial effect were named BSXE-1601.

Example Embodiment

[0048] Example 2 Classification and identification of Bacillus subtilis BSXE-1601

[0049] 1. Morphological characteristics

[0050] The characteristics of a single colony of Bacillus subtilis BSXE-1601 in a nutrient agar medium are: round or irregular shape, irregular colony edges, opaque lawn, off-white, moist surface, easy to pick. Gram staining and microscopic examination showed that the bacterium is Gram-positive, rod-shaped, spore-producing, and spores are mesophytic and oval.

[0051] 2. Molecular genetics characteristics

[0052] The DNA of Bacillus subtilis BSXE-1601 was extracted, and the 16S rDNA region was amplified and sequenced. The results showed that it was highly similar to the Bacillus subtilis sequence (see the instructions attached for details) figure 1 ), and finally determined that it belongs to the genus Bacillus subtilis.

[0053] 3. Physiological and biochemical characteristics

[0054] The physiological and biochemical characteristics of Bacillus subtilis BSXE-...

Example Embodiment

[0057] Example 3 Determination of growth conditions of Bacillus subtilis BSXE-1601

[0058] 1. Take the Bacillus subtilis BSXE-1601 bacterial solution, inoculate it in nutrient broth medium, and culture it at 22℃, 24℃, 26℃, 28℃ and 30℃ to determine its growth.

[0059] 2. Take the Bacillus subtilis BSXE-1601 bacterial solution and inoculate it into nutrient broth with pH values ​​of 6.5, 7.0, 7.5, 8.0 and 8.5 respectively, and culture it at 28°C to determine its growth.

[0060] 3. Take the Bacillus subtilis BSXE-1601 bacterial solution, inoculate it in the nutrient broth medium, cultivate it under the conditions of rotation speed of 70r / min, 100r / min, 130r / min, 160r / min and 190r / min, and determine respectively Its growth.

[0061] The result shows: as attached Figure 2-4 As shown, Bacillus subtilis BSXE-1601 grows best at 28°C, pH 7.0, and rotation speed 190r / min.

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Abstract

The invention relates to wide-spectrum bacillus subtilis BSXE-1601 for inhibiting aquatic product pathogenic vibrio, and further relates to a compound bacterial preparation taking the bacillus subtilis as the main ingredient. Meanwhile, the invention also relates to a preparation method of the compound bacterial preparation by taking the bacillus subtilis as the main ingredient. The bacillus subtilis BSXE-1601 is preserved in China General Microbiological Culture Collection Center (CGMCC), the culture preservation number is CGMCC NO. 15949, and the preservation data is June 19th, 2018. The bacillus subtilis can effectively inhibit growth of various kinds of vibrios such as vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus and vibrio harveyi, meanwhile can promote growth of farmed animals to some extent, and improves the survival rate and bait utilization rate.

Description

technical field [0001] The patent of the present invention belongs to the field of microorganisms and their applications, and in particular relates to a strain of Bacillus subtilis (Bacillus subtilis) BSXE-1601 that broadly inhibits aquatic pathogenic Vibrio; -1601 as the main component of the composite bacteria preparation; meanwhile, the present invention also relates to a preparation method of the above-mentioned Bacillus subtilis (Bacillus subtilis) BSXE-1601 as the main component of the composite bacteria preparation. Background technique [0002] Vibriosis is caused by bacteria of the genus Vibrio and is an important disease in aquaculture activities. Vibrio disease is characterized by strong explosiveness and high mortality, and can occur in a variety of aquaculture animals, such as fish, shellfish and crustaceans. In fish farming, Vibrio alginolyticus and Vibrio parahaemolyticus were the main culprits responsible for the massive death of large yellow croakers in cag...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/125C12R1/085
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 田相利蒋雯雯王栋栋李海东赵坤蔡玉勇李超
Owner OCEAN UNIV OF CHINA
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