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Method improving disinfecting and sterilizing effect of tetrastlgma hemsleyanum explant

A technology for disinfection and explants, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve problems such as low survival rate, high contamination rate of clover explants, and difficulties in production practice and application

Inactive Publication Date: 2018-11-27
SHANGRAO NORMAL UNIV +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

At present, using conventional disinfection and sterilization methods, the contamination rate of the explants of Clover is high, the survival rate is low, and the growth rate is slow, resulting in low production efficiency and difficult production practice.

Method used

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Effect test

Embodiment Construction

[0004] The present invention will be further described in conjunction with following embodiment:

[0005] (1) First processing

[0006] Take out the trefoil explant, and soak it in solution 1: 0.3-0.5g / L carbon nanotube;

[0007] (2) The second treatment

[0008] After soaking in culture solution 1 for 0.5h, soak the trifolium explants in solution 2: 0.3-0.5g / L graphene quantum dots;

[0009] (3) The third treatment

[0010] After soaking in the culture solution 2 for 0.5h, the trifolium explants were soaked in the solution 3: 0.3-0.5g / L carbon nanotubes + 0.3-0.5g / L graphene quantum dots;

[0011] (4) The fourth treatment

[0012] After soaking in culture solution 3 for 0.5h, transfer the trefoil explants to a clean bench and soak in solution 4: 0.3-0.5g / L carbon nanotubes + 0.3-0.5g / L graphene quantum dots +70%-75% alcohol, disinfected according to the regular time;

[0013] (5) The fifth treatment

[0014] After washing according to conventional methods, soak the clo...

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PUM

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Abstract

The invention provides a method for improving the disinfecting and sterilizing effect of a tetrastlgma hemsleyanum explant. The method comprises the step of sequentially treating a tetrastlgma hemsleyanum explant with seven solutions or culture solutions containing carbon nano-tubes or graphene quantum dots so as to remarkably reduce the pollution rate of the tetrastlgma hemsleyanum explant. Compared with the prior art, the tetrastlgma hemsleyanum explant has high survival rate, small pollution rate and high growing speed after being disinfected and sterilized.

Description

Technical field: [0001] The invention relates to a method for improving the disinfection and sterilization effect of plant explants, in particular to a method for improving the disinfection and sterilization effect of A. trifoliate explants. Background technique: [0002] Three-leaf green (Tetrastigma hemsleyanum Diels et Gilg), the scientific name is three-leaf climbing vine, and it is commonly known as golden thread hanging gourd, three-leaf pair, three-leaf flat rattan, broken stone beads, thunder gall, and a bead. The root is called snake Aconite, etc. are perennial vines of the genus Cliffaceae of the grape family, and are unique and rare Chinese medicinal plants in my country. Clover is mainly distributed in my country's Zhejiang, Hunan, Jiangxi, Fujian, Guangxi, Chongqing, Hubei, Sichuan, Guangdong, Guizhou and other places. Bottom; Clover is suitable for humid subtropical monsoon and temperate monsoon climates, where the rainfall needs to exceed 800mm, the annual aver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 尹明华陈荣华谢妮妮
Owner SHANGRAO NORMAL UNIV
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