Method for quickly detecting metrafenone in water by adopting liquid chromatography-mass spectrometry technology

Abstract

The invention relates to a detecting method for residual amount of bactericide in water and specifically relates to a method for quickly detecting metrafenone in water by adopting a liquid chromatography-mass spectrometry technology. The method mainly comprises the following steps: sample pretreatment, liquid chromatogram separation and mass spectrometric detection. The specific processes of the method are as follows: filtering and purifying a sample; performing HLB column purification; gathering nitrogen; blow-drying and redissolving with acetonitrile water mixed liquor; by adopting SHIM-PACKXRODSII75L*2.0 high performance liquid chromatography as an analytical column, and taking acetonitrile-0.1% formic acid aqueous solution as a flowing phase, performing gradient elution at flow rate of 0.3ml / min; separating and detecting metrafenone in water under the mode of positive ion electrospray ionization and the mode of multi-reaction monitoring; optimizing mass spectrometry conditions andcreating optimal mass spectrometry conditions, thereby achieving the highest sensitivity of metrafenone. The detecting method has the characteristics of simple sample pretreatment steps, high extraction ratio, high accuracy, excellent purifying effect, less detection time consumption and low cost.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and food safety, in particular to a method for rapidly detecting metrafenone in water by using liquid mass spectrometry technology. Background technique [0002] Metrafenone, also known as BASF fungicide, is a benzophenone fungicide developed by BASF in Germany. This agent is mainly used for the prevention and treatment of powdery mildew and eye spot diseases in crops such as cereals, grapes and cucumbers. It interferes with the germination of pathogenic spores. The development and formation of appressoria inhibit the spore germination of powdery mildew and control the spread of the disease. At the same time, by interfering with the establishment and formation of polar actin tissue, the formation of the top cells of the mycelium of the pathogen is disturbed and inhibited, thereby It hinders the normal development and growth of mycelium, inhibits and hinders the invasion of powdery mildew bacte...

AI Technical Summary

Problems solved by technology

Because of its unique mechanism of action and good bactericidal effect, it is more and more widely used, but it has low toxicity, acute or long-term harm to the aquatic environment, has an impact on the growth of fish, algae, and microorganisms in the water, and has potential harm to animals, plants, and humans.

[0003] At present, there are few analysis and detection methods for this drug in China. For the purpose of monitoring the water environment and protecting the ecological environment, it is necessary to research and design a new type of detection method for the residual amount of fungicides in water, which can fill the domestic liquid quality. method to detect the blank of metrafenone in water, and provide a better and feasible method reference for relevant water quality control and inspection departments

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