Polypeptide specifically binding to Mycoplasma pneumoniae positive serum and diagnostic kit

A technology of mycoplasma pneumoniae and diagnostic kits, applied in peptides, instruments, analytical materials, etc., can solve the problems of low specificity, cumbersome operation, high cost, etc., and achieve high sensitivity and specificity, high reliability, and easy The effect of the operation

Active Publication Date: 2022-07-19
CHENZHOU NO 1 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These previous studies were based on traditional protein expression and purification, which was cumbersome, expensive and low specificity

Method used

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  • Polypeptide specifically binding to Mycoplasma pneumoniae positive serum and diagnostic kit
  • Polypeptide specifically binding to Mycoplasma pneumoniae positive serum and diagnostic kit
  • Polypeptide specifically binding to Mycoplasma pneumoniae positive serum and diagnostic kit

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Embodiment Construction

[0018] The present invention is described in detail below in conjunction with specific experimental steps, but the protection scope of the present invention is not limited to the following examples.

[0019] In the following experiments, the purified Mycoplasma pneumoniae positive mixed serum was used as the target molecule, and the phage display random 7-peptide library was biopanned, and the phages that could specifically bind to the Mycoplasma pneumoniae positive mixed serum were screened. As a coating antigen, the reaction of phage and exogenous polypeptides with different clinical sera was detected to prove the application value of the phage and its polypeptides in the serological diagnosis of Mycoplasma pneumoniae.

[0020] Materials and methods: The positive mixed serum and negative mixed serum (from Chenzhou First People's Hospital) of patients infected with Mycoplasma pneumoniae were mixed with saturated (NH4) 2 SO 4 Purification method: add equal volume of normal sa...

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Abstract

The present invention provides a polypeptide that can specifically bind to Mycoplasma pneumoniae positive serum, and the amino acid sequence of the polypeptide is shown in SEQ ID NO: 1: T-V-N-F-K-L-Y. The present invention also provides a serological diagnostic kit for Mycoplasma pneumoniae infection, which contains the above-mentioned polypeptide specifically binding to Mycoplasma pneumoniae positive serum. In addition, the present invention also provides a phage-displayed polypeptide-based diagnostic kit for Mycoplasma pneumoniae, the kit includes a core phage, wherein the core phage is a phage displaying the above-mentioned polypeptide. The polypeptide and its corresponding core phage provided by the present invention have high sensitivity and specificity, and have good clinical diagnostic value.

Description

technical field [0001] The present invention provides a polypeptide that can specifically bind to Mycoplasma pneumoniae positive serum, and a diagnostic kit based on the polypeptide, and more relates to a Mycoplasma pneumoniae diagnostic kit based on a phage display polypeptide, which belongs to the field of biological preparations. Background technique [0002] Mycoplasma pneumoniae (MP) is a prokaryotic organism between viruses and bacteria. The pathological changes caused by its infection are mainly interstitial pneumonia, accounting for about 1 / 2 of non-bacterial pneumonia in humans and animals. . MP is highly contagious, especially easy to spread to children, and is the main pathogen of community-acquired pneumonia, so the early diagnosis of MP pneumonia is extremely important. At present, Mycoplasma pneumoniae infection is mainly diagnosed by MP isolation and culture, serological detection, PCR detection and physical diagnosis. There is still a lack of sensitive and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06G01N33/569
CPCG01N33/56933C07K7/06
Inventor 史文元曾焱华赵兰华徐玉娟李胜涛朱军民徐桂珍李莉萍彭小友
Owner CHENZHOU NO 1 PEOPLES HOSPITAL
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