Method for detecting microbial limit of rhubarb powder

A technology of microbial limit and detection method, applied in the detection field of traditional Chinese medicine, can solve the problems such as no record of microbial limit inspection method of rhubarb powder, the safety of rhubarb powder cannot be truly guaranteed, and endanger human health, etc., and achieves easy operation, verification accuracy, The effect of ensuring safety

Inactive Publication Date: 2018-12-11
WENZHOU CENT HOSPITAL
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Problems solved by technology

These components have an inhibitory effect on microorganisms at a certain concentration, and may cause varying degrees of damage to microorganisms contaminated in pharmaceuticals
If the conventional detection method is used, the contaminated microorganisms in the test product may not be detected under the influence of these substances, but these suppressed or damaged microorganisms are not dead and can still survive for a long period of time. After the living environment of microorganisms changes, such as when the antiba

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  • Method for detecting microbial limit of rhubarb powder
  • Method for detecting microbial limit of rhubarb powder
  • Method for detecting microbial limit of rhubarb powder

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Embodiment Construction

[0018] A detection method of rhubarb powder microbial limit, comprising the following steps:

[0019] Step 1: Weigh 10 mg of rhubarb powder to be tested with an electronic balance, add sodium chloride-peptone buffer solution with a pH of 7.0 to 100 mL to make a test solution;

[0020] Step 2: Configure medium and buffer, configure tryptone liquid medium, tryptone agar medium, Sabouraud dextrose agar medium, mannitol sodium chloride agar medium according to the provisions of the 2015 edition of "Chinese Pharmacopoeia" , cetyltrimethylammonium bromide agar medium, pH7.0 sodium chloride-peptone buffer;

[0021] Step 3: Put Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis into tryptone soy agar medium for culture respectively. The culture method is 18-24 hours at 33°C. Take 1 platinum ear each, and then dilute it with 0.9% sterile sodium chloride solution to form a bacterial suspension containing 50-100cfu per 1ml; among them, dilute Staphylococcus aureus to 1...

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Abstract

The invention discloses a method for detecting microbial limit of rhubarb powder. First, a rhubarb powder test sample is weighed, and a test solution is configured; then a medium and a buffer solutionrequired by the invention are configured, then a bacterium suspension of five microorganisms of staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida albicans and aspergillus niger is cultured, then membrane-filter method is used for counting and bacterial colony recovery rate is detected, If the bacterial colony recovery rate is greater than 70%, the test data is proved to bevalid, if the bacterial colony recovery rate is less than 70%, the test results are invalid, it is necessary to re-dilute the test solution and then use the membrane-filter method to detect, so thata effective value is measured and obtained, and at the same time, the accuracy of the test results can be verified through a control bacteria test method. The method is accurate, effective, rigorous and easy to operate, and is suitable for measuring the microbial limit of the rhubarb powder to ensure the safety of clinical use of the rhubarb powder.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of rhubarb powder microbial limit. Background technique [0002] Rhubarb is a common traditional Chinese medicine, mainly derived from the dried roots and rhizomes of Rhubarb palmatum, Rhubarb tangutica, or Rheum officinalis of the Polygonaceae plant. Rhubarb is bitter in taste, cold in nature, and returns to the spleen, stomach, large intestine, liver, and pericardium meridians. It has the effects of purging and attacking stagnation, clearing heat and purging fire, cooling blood and detoxifying, expelling blood stasis to stimulate menstruation, promoting dampness and reducing jaundice. [0003] The microbial limit detection of drugs is one of the important indicators to ensure safe drug use. However, due to the characteristics of the test product itself, it may contain some components with antibacterial effects, such as Coptidis and Calcul...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 翁金月金利思郑京胜
Owner WENZHOU CENT HOSPITAL
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