Method for detecting microbial limit of rhubarb powder

Abstract

The invention discloses a method for detecting microbial limit of rhubarb powder. First, a rhubarb powder test sample is weighed, and a test solution is configured; then a medium and a buffer solutionrequired by the invention are configured, then a bacterium suspension of five microorganisms of staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida albicans and aspergillus niger is cultured, then membrane-filter method is used for counting and bacterial colony recovery rate is detected, If the bacterial colony recovery rate is greater than 70%, the test data is proved to bevalid, if the bacterial colony recovery rate is less than 70%, the test results are invalid, it is necessary to re-dilute the test solution and then use the membrane-filter method to detect, so thata effective value is measured and obtained, and at the same time, the accuracy of the test results can be verified through a control bacteria test method. The method is accurate, effective, rigorous and easy to operate, and is suitable for measuring the microbial limit of the rhubarb powder to ensure the safety of clinical use of the rhubarb powder.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of rhubarb powder microbial limit. Background technique [0002] Rhubarb is a common traditional Chinese medicine, mainly derived from the dried roots and rhizomes of Rhubarb palmatum, Rhubarb tangutica, or Rheum officinalis of the Polygonaceae plant. Rhubarb is bitter in taste, cold in nature, and returns to the spleen, stomach, large intestine, liver, and pericardium meridians. It has the effects of purging and attacking stagnation, clearing heat and purging fire, cooling blood and detoxifying, expelling blood stasis to stimulate menstruation, promoting dampness and reducing jaundice. [0003] The microbial limit detection of drugs is one of the important indicators to ensure safe drug use. However, due to the characteristics of the test product itself, it may contain some components with antibacterial effects, such as Coptidis and Calcul...

AI Technical Summary

Problems solved by technology

These components have an inhibitory effect on microorganisms at a certain concentration, and may cause varying degrees of damage to microorganisms contaminated in pharmaceuticals

If the conventional detection method is used, the contaminated microorganisms in the test product may not be detected under the influence of these substances, but these suppressed or damaged microorganisms are not dead and can still survive for a long period of time. After the living environment of microorganisms changes, such as when the antibacterial components are eliminated or the concentration is reduced, these microorganisms can recover and multiply, and patients can also endanger human health after using them. Therefore, it is necessary to establish an accurate detection method for the limit of microorganisms, accurate and effective detection microbial content

However, in various literatures, there is no record about the inspection method of the microbial limit of rhubarb powder, so the safety of rhubarb powder in clinical use cannot be truly guaranteed.

Images