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A miRNA composition for predicting chronic hepatitis B inflammatory damage

A technology for chronic hepatitis B and inflammation, applied in the field of medical testing, can solve problems such as unacceptable by patients, and achieve the effect of improving accuracy

Active Publication Date: 2021-09-17
镇江市第三人民医院
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AI Technical Summary

Problems solved by technology

Although liver histological examination is the gold standard for determining liver tissue injury, it is invasive and limited and cannot be accepted by most patients

Method used

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  • A miRNA composition for predicting chronic hepatitis B inflammatory damage
  • A miRNA composition for predicting chronic hepatitis B inflammatory damage
  • A miRNA composition for predicting chronic hepatitis B inflammatory damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening of chronic hepatitis B inflammatory injury-related miRNA

[0028] Using a four-step screening method:

[0029] (1) In the discovery stage, we used the next-generation deep sequencing technology (IlluminaHiseq 2000) to detect the mixed samples of the discovery set, found miRNAs with differential expression, and selected 6 cases with mild and moderate inflammation and necrosis respectively;

[0030] (2) In the screening stage, use qRT-PCR method to select internal reference miRNA, establish a uniform small number of cohort screening sets, measure the cycle number (Ct) by 2-△△Ct, and eliminate miRNACt value75%; For miRNAs with p<0.05, 20 cases were selected for mild and moderate inflammation and necrosis respectively;

[0031] (3) In the training stage, the target miRNAs selected in the training and screening stage were tested by using qRT-PCR on a large sample training set (136 cases of mild and 201 cases of moderate inflammation and necrosis respecti...

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Abstract

The present invention relates to a miRNA composition for predicting chronic hepatitis B inflammatory damage, said composition comprising hsa-miR-1285-5p, hsa-miR-193a-5p and hsa miR-26a-5p, the nucleotide sequences are respectively See SEQ ID NO. 1-3. The present invention solves the problem of invasiveness and limitations brought about by liver histological examination to evaluate chronic hepatitis B inflammatory injury in the prior art, and detects the above-mentioned miRNA composition by qRT-PCR, which can realize the detection of chronic hepatitis B inflammatory injury For early diagnosis, miRNA as a biological marker is a kind of non-invasive sampling, which can be used to assist in the diagnosis of whether the subject has chronic hepatitis B inflammatory damage through the expression profile of a single miRNA or miRNA composition under non-invasive conditions, which improves the judgment of the disease the accuracy rate.

Description

technical field [0001] The invention relates to a miRNA composition for predicting chronic hepatitis B inflammatory damage, belonging to the technical field of medical testing. Background technique [0002] MicroRNA (microRNA) is a type of endogenous non-coding single-stranded small RNA with a length of 18-24 nucleotides. In recent years, there have been more and more studies on the biological functions of microRNAs, and it has been found that they play an important role in the physiology and pathology of the body. Under physiological conditions, they participate in the process of cell proliferation and differentiation. If the expression and function are out of balance, it may lead to tumors including leukemia. As well as various pathological phenomena such as viral and bacterial infections. [0003] MicroRNAs are ubiquitous in human body fluids, are stable in nature, can be quantitatively detected, and have significant disease specificity. Recent studies have shown that t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q1/706C12Q2600/158C12Q2600/178
Inventor 谭友文陈丽叶云
Owner 镇江市第三人民医院
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