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Application of TSPAN1 in diagnosis, prognosis and treatment of breast cancer metastasis

A breast cancer and bone metastasis technology, applied in the field of biomedicine, can solve problems such as affecting the quality of life and longevity of patients, increasing the economic burden of patients' families, and difficult clinical treatment.

Active Publication Date: 2018-12-11
江门市中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In most cases, breast cancer has already caused bone metastases before causing obvious clinical symptoms, or the primary tumor has not recurred after treatment, but the formation of bone metastases is still found, which has brought great significance to clinical treatment. big difficulty
Once breast cancer has bone metastasis, it indicates that the patient has entered the advanced stage of the tumor, which seriously affects the quality of life and life expectancy of the patient, increases the economic burden of the patient's family, and consumes a lot of medical resources

Method used

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  • Application of TSPAN1 in diagnosis, prognosis and treatment of breast cancer metastasis
  • Application of TSPAN1 in diagnosis, prognosis and treatment of breast cancer metastasis
  • Application of TSPAN1 in diagnosis, prognosis and treatment of breast cancer metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1, TSPAN1 is highly expressed in bone metastasis tissues and cells of breast cancer

[0059] In the collected tissue samples and cell lines, the mRNA and protein levels of TSPAN1 were detected by fluorescent quantitative PCR and Western blotting, and the results were as follows: figure 1 As shown, compared with breast cancer tissues without bone metastasis, the mRNA and protein levels of TSPAN1 in breast cancer tissues with bone metastasis increased ( figure 1 A and figure 1 B), compared with human normal breast epithelial cell lines, the mRNA and protein content of TSPAN1 in breast cancer cell lines increased, and the mRNA and protein content of TSPAN1 increased more significantly in metastatic breast cancer cell lines, especially MCF-7 and MDA-MB-231 cell line ( figure 1 C and figure 1 D).

[0060] The above results show that: TSPAN1 is highly expressed in breast cancer bone metastasis tissues and cells. Therefore, the occurrence of breast cancer bone meta...

Embodiment 2

[0061] Example 2. High expression of TSPAN1 indicates short survival time without bone metastasis

[0062] 239 breast cancer tissue samples without bone metastasis were divided into TSPAN1 high-expression group and TSPAN1 low-expression group according to the median TSPAN1 expression level, and the bone metastasis-free survival time was further analyzed in combination with the patient follow-up data of breast cancer tissue samples. The results are as follows: figure 2 As shown, the bone metastasis-free survival time of the TSPAN1 high expression group was longer.

[0063] The above results show that: high expression of TSPAN1 indicates a short bone metastasis-free survival time. Therefore, by detecting the expression level of TSPAN1, the bone metastasis-free survival time can be used as a prognostic indicator to predict whether there is a tendency to bone metastasis of breast cancer; The expression level of TSPAN1 mRNA or protein in breast cancer tissue samples of patients is...

Embodiment 3

[0064] Example 3, downregulation of TSPAN1 gene expression can inhibit breast cancer cell migration and invasion

[0065] MDA-MB-231 or MCF-7 cells were transfected with RNA interference plasmids and control plasmids capable of down-regulating TSPAN1 gene expression, and the results were as follows image 3 As shown, the mRNA and protein expression of TSPAN1 gene in MDA-MB-231 and MCF-7 cells transfected with RNA interference plasmid were significantly down-regulated.

[0066] The transfected MDA-MB-231 and MCF-7 cells were subjected to invasion and migration experiments, and the results Figure 4 As shown, the migration and invasion abilities of MDA-MB-231 and MCF-7 cells transfected with RNA interference plasmids were significantly inhibited.

[0067] The above results indicated that down-regulating the expression of TSPAN1 gene can inhibit the migration and invasion of breast cancer cells.

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Abstract

The invention discloses an application of TSPAN1 in diagnosis, prognosis and treatment of breast cancer metastasis. The invention finds the high expression of TSPAN1 in tissues and cells of breast cancer metastasis and the high expression indicates shorter survival time without osseous metastasis; in vivo and in vitro experiments prove that the down-regulated expression of TSPAN1 genes can restrain the breast cancer metastasis and invasion so as to restrain the osseous metastasis of breast cancer; on the basis of the finding, the invention provides application of a reagent for detecting the expression level of the TSPAN1 gene in preparing a kit for detecting if breast cancer metastasis occurs and / or a prognosis kit for forecasting the breast cancer metastasis trend; a TSPAN1 inhibiting reagent is used for preparing the drug for preventing and / or treating breast cancer metastasis, and a new thought is supplied for diagnosis, prognosis and treatment of breast cancer metastasis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to the application of TSPAN1 in the diagnosis, prognosis and treatment of breast cancer metastasis. Background technique [0002] Metastasis is the main cause of death in cancer patients, and more than 90% of tumor-related deaths are related to distant organ metastasis. Bone is one of the most common distant metastatic organs of solid tumors. Bone-related complications, such as pain, pathological fractures, hypercalcemia, spinal cord compression, and loss of motor function, are accompanied by bone metastasis of tumors. eventually lead to the death of the patient. The current treatment for bone metastasis is palliative. Whether it is surgery, radiotherapy or drug treatment, it only relieves and improves the corresponding symptoms, and cannot significantly improve the survival expectancy of patients. Therefore, once bone metastasis occurs, it not only seriously affects the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00A61P15/14
CPCA61K45/00A61P15/14A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158G01N33/57415G01N33/57484G01N2333/47
Inventor 任栋李宇明杨春潇王斌张鑫
Owner 江门市中心医院
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