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Method for identifying 28 good varieties of daucus carota, and special primer group thereof

An excellent variety, carrot technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/inspection, etc., can solve problems such as application research of unseen SSR markers

Active Publication Date: 2018-12-11
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the application of SSR markers in the specific identification of carrot varieties

Method used

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  • Method for identifying 28 good varieties of daucus carota, and special primer group thereof
  • Method for identifying 28 good varieties of daucus carota, and special primer group thereof
  • Method for identifying 28 good varieties of daucus carota, and special primer group thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Embodiment 1, be used for the development of the SSR primer of detection carrot fine variety

[0138] 1. Take the sand, sterilize at 130-170°C for 2 hours, and then pass it through a standard sieve (2.0mm in diameter) to collect the sand with a diameter below 2.0mm (that is, the sand under the standard sieve).

[0139] 2. After completing step 1, put the collected sand in the seed box, plant the seeds of Dongyang Qicun, and finally put the seed box in the incubator, and cultivate it at 25°C under alternating light and dark conditions. After 14 days, collect 30 seedlings of equal quality Mix the samples to get sample 1.

[0140] According to the above method, replace Dongyang Qicun with Dongyang Xinxiu, Qishan Black Crown, Qishan Zihuangguan, Qishan Caiguan, Sheng-Qihongguan, Qishan Super Three Reds, Super Eight-inch Ginseng, Three Red Seven Inch Ginseng, Touxin Red, Pheasant Red, Super Red Crown Eight Inch Ginseng, Huayu No. 1, South Korea Jinhongfu, Osaka Three Red Se...

Embodiment 2

[0145] Embodiment 2, using the SSR primers developed in Example 1 to detect 28 fine varieties of carrots

[0146] 1. Extraction of Carrot Genomic DNA

[0147] A simple method for extracting DNA using improved SDS (Li Li, Zheng Xiaoying. The establishment of EST-SSR composite markers for the identification of Chinese cabbage and Chinese cabbage varieties. Acta Horticultural Science, 2009, 37 (11): 1627-1634) Extraction Example 1 Samples in (sample1, sample2, sample3, sample4, sample5, sample6, sample7, sample8, sample9, sample10, sample11, sample12, sample13, sample14, sample15, sample 16. Genomic DNA of sample 17, sample 18, sample 19, sample 20, sample 21, sample 22, sample 23, sample 24, sample 25, sample 26, sample 27 or sample 28), to obtain the genomic DNA of the sample.

[0148] 2. PCR amplification

[0149] Using the genomic DNA of the sample as a template, perform PCR amplification with primer pair 2-7A, primer pair 2-10H, primer pair SSR-I, primer pair FJ816243 or p...

Embodiment 3

[0169] Embodiment 3, accuracy experiment

[0170] The 13 seeds to be tested are the seeds of excellent carrot varieties that have been phenotyped.

[0171] See columns 1 to 4 in Table 4 for the name, delivery year, delivery company, and phenotypic identification information of each seed to be tested.

[0172] Table 4. Basic information of good carrot seeds to be tested

[0173]

[0174] 1. Obtaining samples to be tested

[0175] (1) Take the sand, sterilize it at 130-170°C for 2 hours, then pass it through a standard sieve (2.0mm in diameter) to collect the sand with a diameter below 2.0mm (that is, the sand under the standard sieve).

[0176] (2) After completing step (1), put the collected sand in the seed box, plant the seeds to be tested respectively, and finally place the seed box in the incubator, and cultivate at 25°C under alternating light and dark conditions. After 14 days, 30 seedlings were collected. Mix the samples with equal mass to obtain the corresponding...

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Abstract

The invention discloses a method for identifying 28 good varieties of daucus carota, and a special primer group thereof. The primer group is composed of a primer pair 2-7A, a primer pair SSR-1, a primer pair FJ816243, a primer pair 2-10H and a primer pair FJ816249. The primer pair 2-7A is composed of single-chain DNA molecules as shown in the sequence 1 and the sequence 2 in the sequence table. The primer pair 2-10H is composed of single-chain DNA molecules as shown in the sequence 3 and the sequence 4 in the sequence table. The primer pair FJ816243 is composed of single-chain DNA molecules asshown in the sequence 5 and the sequence 6 in the sequence table. The primer pair FJ816249 is composed of single-chain DNA molecules as shown in the sequence 7 and the sequence 8 in the sequence table. The primer pair SSR-1 is composed of single-chain DNA molecules as shown in the sequence 9 and the sequence 10 in the sequence table. The primer group can detect whether to-be-detected daucus carota seeds are the good varieties of the daucus carota or not, thereby having important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying 28 excellent varieties of carrots and a special primer set thereof. Background technique [0002] Species and variety identification is an important part of the quality inspection of seeds, and its main purpose is to determine the degree of conformity between the submitted samples and the declared species or varieties. With the increase in the trade volume of the seed market in recent years, domestic seed operators have one-sidedly pursued economic benefits while ignoring quality, and used other varieties to adulterate and fake, which not only caused economic losses, but also caused confusion in the varieties on the market, making it difficult to distinguish the authentic from the fake, making the real The excellent varieties cannot be effectively protected, which shortens the useful life of the varieties. Therefore, accurate, reliable, fast and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 李丽徐秀兰赵佳惠吴萍梁毅李晨宋顺华卢艳马连平
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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