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Special culture dish for cardiomyocytes and nerve cells

A technology for nerve cells and cardiomyocytes, which is applied in the cultivation devices of tissue cells/viruses, biochemical equipment and methods, and biochemical instruments, etc., can solve the problems of lack of high borosilicate glass culture dishes, etc. Good light and good adsorption effect

Active Publication Date: 2018-12-14
太原市罗塞塔石生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problem of lacking a high borosilicate glass petri dish with simple pretreatment and reliable structure and strong adsorption force

Method used

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  • Special culture dish for cardiomyocytes and nerve cells
  • Special culture dish for cardiomyocytes and nerve cells
  • Special culture dish for cardiomyocytes and nerve cells

Examples

Experimental program
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Effect test

Embodiment 1

[0030] S1, in high-purity NH at a pressure of 5Pa 3 Under ambient conditions, vacuum plasma treatment for 10 minutes, and then vacuum freeze-drying for 1 minute;

[0031] S2, grafted O at a pressure of 30Pa 2 Under ambient conditions, vacuum plasma treatment for 4 minutes, and then vacuum freeze-drying for 10 minutes.

Embodiment 2

[0033] S1, high-purity NH at a pressure of 100Pa 3 Under ambient conditions, vacuum plasma treatment for 4 minutes, and then vacuum freeze-drying for 7 minutes;

[0034] S2, grafted O at a pressure of 70Pa 2 Under ambient conditions, vacuum plasma treatment for 7 minutes, and then vacuum freeze-drying for 1 minute.

Embodiment 3

[0036] S1, in high-purity NH at a pressure of 30Pa 3 Under ambient conditions, vacuum plasma treatment for 7 minutes, and then vacuum freeze-drying for 4 minutes;

[0037]S2, grafted O at a pressure of 5Pa 2 Under ambient conditions, vacuum plasma treatment for 1 min, and then vacuum freeze-drying for 7 min.

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Abstract

The invention specifically discloses a special culture dish for cardiomyocytes and nerve cells, belonging to the technical field of cell culture devices. According to a technical scheme in the invention, the special culture dish comprises a barrel-shaped dish body; a through hole is vertically formed in the bottom of the dish body; and the lower end of the through hole is hermetically bonded witha piece of high borosilicate glass with a thickness of 0.13-0.175 mm. After bonding assembling of the dish body and the high borosilicate glass, the following two steps are carried out for modification: step 1, carrying out vacuum plasma treatment in a high-purity NH3 environment with a pressure of 5-100 Pa for 1-10 min, and then carrying out vacuum drying for 1-10 min; and step 2, carrying out vacuum plasma treatment in a grafting O2 environment with a pressure of 5-100 Pa for 1-10min, and then carrying out vacuum drying for 1-10 min.

Description

technical field [0001] The invention relates to the technical field of cell culture devices, in particular to a special culture dish for cardiomyocytes and nerve cells. Background technique [0002] Cell culture is firstly the growth of single cells under in vitro conditions. It is widely used in biology, bioengineering, drug development, clinical and other fields. Now it has become one of the most effective methods in biology, medicine and pharmaceutical research. Cardiomyocytes cultured in vitro have the characteristics of spontaneous rhythm and contraction, making the culture of cardiomyocytes one of the basic methods and means for the study of heart diseases. The culture of nerve cells is of great significance to the treatment and research of damaged nerve tissue. [0003] In traditional cell culture methods, petri dishes generally use SiO 2 glass, SiO 2 Glass has strong adsorption to cells, but due to its poor light transmission, it is difficult to observe the growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M23/02C12M23/20
Inventor 石鹏程张蓉王瑞
Owner 太原市罗塞塔石生物技术有限公司
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