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Up-regulation long-chain non-coding RNA markers for lung cancer and application thereof

A marker, non-coding technology, applied in the field of biomedicine, can solve the problems of lung injury, risk, sensitivity and specificity need to be improved, etc., and achieve the effect of high accuracy and high sensitivity

Active Publication Date: 2018-12-14
FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity of ultrasound diagnosis is low, and lung puncture will damage the lungs of patients, which is risky and difficult to promote. As a result, many patients are not diagnosed until the decompensated stage of lung cancer
Recent studies have found that serum metabolic markers can be used for the diagnosis of lung cancer, but their sensitivity and specificity need to be improved, and they have not been clinically used as indicators for the diagnosis of lung cancer.

Method used

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  • Up-regulation long-chain non-coding RNA markers for lung cancer and application thereof
  • Up-regulation long-chain non-coding RNA markers for lung cancer and application thereof
  • Up-regulation long-chain non-coding RNA markers for lung cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Lung cancer diagnostic markers:

[0031] There are 11 kinds of lung cancer markers provided by the present invention, including: AC089998.4, AC103925.1, AC113189.2, AC130324.2, AC239868.1, AL031985.3, AL079303.1, AL121902.1, AP000873.3, PCAT6 , PVT1; the nucleotide probe sequences of these 11 markers are shown in Table 1:

[0032] Table 1. Nucleotide probe sequences for 11 markers

[0033] landmark

Embodiment 2

[0034] Example 2: Screening and identification of diagnostic markers for lung cancer

[0035] In the present invention, gene chips or real-time fluorescent quantitative PCR methods are used to screen and identify markers, but the identification is not necessarily limited to the above methods, and other methods such as chemical analysis methods are also suitable for screening and identification of markers. In the present invention, lung tissue is taken as an example to illustrate the screening and identification of markers:

[0036]1. Obtain subject samples and perform pretreatment:

[0037] Lung tumor tissues and normal lung tissues of 23 patients with lung cancer were surgically extracted, and total RNA in tissue blocks or cells was extracted using Trizol (Invitrogen, USA). and further adopt The RNA clean-up kit (Macherey Nagel, Germany) was used to purify the total RNA through a column. Quantification was then performed by spectrophotometric methods and integrity was ch...

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Abstract

The invention discloses up-regulation long-chain non-coding RNA markers for lung cancer and an application thereof. The markers are combinations of AC089998.4, AC103925.1, AC113189.2, AC130324.2, AC239868.1, AL031985.3, AL079303.1, AL121902.1, AP000873.3, PCAT6 and PVT1. The markers can be used alone or in combination of two kinds or in combination of more than two kinds for early diagnosis of lung cancer. 11 lung cancer markers provided by the invention have the characteristics of high accuracy and high sensitivity. All the areas AUC of the 11 markers under a prediction line are larger than 0.9, and the 11 markers are suggested to have the characteristics of high accuracy and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a lung cancer up-regulated long-chain non-coding RNA marker and its application. Background technique [0002] Lung cancer is the malignant tumor with the highest incidence rate in China and the world, and it is also the leading cause of cancer death in men. The incidence of lung cancer in the female population is second only to breast cancer. Global Burden of Disease (GBD) data shows that in 2016, the number of people suffering from trachea, bronchus or lung cancer in the world exceeded 2.8 million, of which the number of patients in China was as high as 1 million. In 2016, the number of deaths from the above-mentioned cancers worldwide was 1.7 million, accounting for 3.12% of the total deaths. In 2016, the number of patients who died in China was 590,000, accounting for 6.11% of the total number of deaths. Statistics show that from 1990 to 2016, the global pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/178
Inventor 张艳亮李文兴段勇单斌
Owner FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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