A kind of high-purity odorless phycocyanin and its preparation method and application

A high-purity phycocyanin technology, which is applied in the field of separation and purification of phycocyanin, can solve the problems of unsuitability for industrial production, long cycle time, and fishy smell of the product, and achieve improved leaching rate and leaching amount, short extraction cycle, and pigment destruction. small effect

Active Publication Date: 2019-11-08
HENAN ZHONGDA HENGYUAN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to overcome the deficiencies and shortcomings of phycocyanin in the prior art, such as low extraction efficiency, long cycle, high purification cost, cumbersome operation, unsuitability for industrial production, and fishy smell of the product, the primary purpose of the present invention is to provide a high-purity non-fishy Preparation method of phycocyanin

Method used

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  • A kind of high-purity odorless phycocyanin and its preparation method and application
  • A kind of high-purity odorless phycocyanin and its preparation method and application
  • A kind of high-purity odorless phycocyanin and its preparation method and application

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preparation example Construction

[0073] A preparation method of high-purity odorless phycocyanin, comprising the following steps:

[0074] (1) uniformly mix spirulina and water, disperse at 10-25°C for 2-5 hours, and obtain a spirulina dispersion;

[0075] (2) The spirulina dispersion prepared in step (1) is prepared as a phosphate buffer containing spirulina and the final concentration of phosphate is 0.05~0.12mol / L, under stirring conditions, leaching at 10~25°C for 12~ 18h, obtain spirulina extract;

[0076] (3) adding 0.5~3% of the spirulina extract quality and 8~15% disodium hydrogen phosphate solution in the spirulina extract obtained in step (2), mix uniformly; then add the spirulina Calcium chloride solution with a mass concentration of 2 to 10% and a mass concentration of 15 to 25% is stirred for 10 to 20 minutes; after the stirring is completed and the pH of the system is stable, the pH of the system is adjusted to 7.0 to 7.5 with lye to obtain flocculation After the extract;

[0077] (4) adding ...

Embodiment 1

[0119] (1) Add 46kg of reverse osmosis membrane filter water into a 100L extraction tank, then slowly add 1.5kg of spirulina dry powder into the extraction tank, stir to make it evenly dispersed; then maintain the system at 20°C, stir and disperse for 3 hours to obtain a spirulina dispersion ;

[0120] (2) add disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 300.9g and sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 (2) 586.7g, containing spirulina and a phosphate buffer solution with a final concentration of phosphate radicals of 0.1mol / L, under stirring conditions, leaching at 20°C for 15h to obtain a spirulina extract;

[0121] (3) Adding 483.9g mass concentration to the spirulina extract prepared in step (2) is 10% disodium hydrogen phosphate solution, stirs for 8min, makes it mix uniformly; Adds 2903.3g mass concentration 20% chloride Calcium solution, stirred for 15 minutes; after the stirring was completed and the pH value of the system was stable, the pH value of th...

Embodiment 2

[0128] (1) Add 46kg of reverse osmosis membrane filter water into a 100L extraction tank, then slowly add 1.7kg of spirulina dry powder into the extraction tank, stir to make it evenly dispersed; then maintain the system at 22°C, stir and disperse for 2 hours to obtain a spirulina dispersion ;

[0129] (2) add disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 179.1g and sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 (2) 349.5g, obtain the phosphate buffer that contains spirulina and the final concentration of phosphate is 0.06mol / L, under stirring condition, 18 ℃ of leaching 18h, obtain spirulina extract;

[0130] (3) Adding 241.1g mass concentration to the spirulina extract obtained in step (2) is a 15% disodium hydrogen phosphate solution, stirs for 5min, and makes it mix uniformly; then adds 4822.9g mass concentration 15% chloride Calcium solution, stirred for 10 minutes; after the stirring was completed and the pH value of the system was stable, the pH value of the syst...

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Abstract

The invention belongs to the technical field of separation and purification of spirulina blue colors, and concretely relates to a high-purity odorless spirulina blue color, and a preparation method and an application thereof. The high-purity odorless spirulina blue color is obtained by the steps of dispersion, extraction, flocculation, filtration, purification, concentration, deodorization and concentration. Water extraction and phosphate buffer solution extraction modes are adopted, and the osmotic pressure of a system is increased stepwise to stepwise improve the extraction rate and the extraction amount of phycocyanins, the whole extraction and flocculation process does not require low temperature, ultrasonication or high-pressure homogenization, so the method has the advantages of mildconditions, low damages to the color, short extraction cycle, no excessive introduction of other ions or components, removal of calcium ions and phosphate ions by flocculation, and protection of thephycocyanins in the presence of sodium ions and chloride ions; the high-purity odorless spirulina blue color can be well removed by subsequent membrane separation, and belongs to a processing aid allowed in foods. The obtained high-purity odorless spirulina blue color has the advantages of high purity, high color value, high yield and odorlessness.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of phycocyanin, and in particular relates to a high-purity odorless phycocyanin and a preparation method and application thereof. Background technique [0002] Spirulina, also known as Arthrospira or cyanobacteria, belongs to the phylum Cyanophyta, mainly including Spirulina maxima, Spirulina pure top and Spirulina spirulina. Spirulina is extremely rich in nutrients. It contains a lot of protein, multivitamins and minerals. It can be used as a high-protein, low-fat, low-cholesterol, and low-calorie health food. It is recommended by the United Nations Food and Agriculture Organization as "the most ideal human tomorrow" food". As spirulina is increasingly being developed as high-protein functional food, health products, etc., the development and utilization of spirulina will also have a broader prospect. [0003] Phycocyanin is extracted from algal cells containing phycobilisom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/795C07K1/14C07K1/34C07K1/30C07K1/36
CPCC07K14/795
Inventor 金子恒潘天义文雁君李林正
Owner HENAN ZHONGDA HENGYUAN BIOTECH CO LTD
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