Microbial inoculum with high salt tolerance and oil degradation rate and preparation method thereof

A microbial agent and salt-tolerant technology, applied in the field of microbial agent and its preparation, can solve the problems of poor product maturity, long fermentation cycle, low degradation rate of food waste oil, etc., and achieve the effect of reducing purchase cost

Active Publication Date: 2022-07-19
苏州微宏生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a microbial agent with high salt resistance and oil degradation rate and its preparation method, which can solve the problems of high salt content in kitchen waste, low food waste oil degradation rate, long fermentation cycle, and product failure. The problem of poor maturity

Method used

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  • Microbial inoculum with high salt tolerance and oil degradation rate and preparation method thereof
  • Microbial inoculum with high salt tolerance and oil degradation rate and preparation method thereof
  • Microbial inoculum with high salt tolerance and oil degradation rate and preparation method thereof

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preparation example Construction

[0038] See figure 1 , the preparation method of the microbial inoculum with high salt tolerance and grease degradation rate of the present invention comprises the following steps:

[0039] S1. Provide strain raw materials and culture medium, strain materials include Bacillus phlei, Bacillus amyloliquefaciens, Lactobacillus plantarum, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces alba and Aspergillus oryzae; culture medium includes solid medium, liquid Culture medium and fermentation medium, wherein the solid medium includes beef extract peptone agar medium, potato dextrose agar medium and lactic acid bacteria solid medium; liquid medium includes beef extract peptone liquid medium, potato dextrose liquid medium and lactic acid Bacterial liquid culture medium; fermentation medium is: molasses 3%, corn steep liquor powder 8%, magnesium sulfate 0.2%, sodium hydroxide 0.15%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%, and the balance is w...

Embodiment 1

[0049] S1. Slope culture: under sterile conditions, inoculate Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus megaterium in beef extract peptone agar medium for cultivation, and inoculate plant lactic acid bacteria in lactic acid bacteria solid medium (MRS) Cultivate, inoculate Saccharomyces cerevisiae, Streptomyces alba and Aspergillus oryzae in potato dextrose agar medium (PDA) for cultivation, wherein, plant lactic acid bacteria, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces alba, Aspergillus oryzae , Bacillus subtilis and Bacillus amyloliquefaciens were cultured at 30℃ for 2 days;

[0050] S2, primary seed culture: the strains cultivated in step S1 are individually picked and inoculated into 50mL / 250mL liquid culture medium under sterile conditions, plant lactobacillus, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces alba, rice Aspergillus was cultured for 2 days under the condition of 30℃, 160r / min shaker, Bacillus subtilis and Bacillu...

Embodiment 2

[0056] The preparation method of the present embodiment is basically similar to that of the first embodiment, and the difference lies in that the inoculation amount in step S3 is changed from 5% to 4% to obtain the high-salt and high-fat kitchen waste treatment microbial inoculum of the present embodiment. The bacterial composition is:

[0057]

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Abstract

The invention relates to a microbial inoculum with high salt tolerance and oil degradation rate and a preparation method thereof. The microbial inoculum is prepared by slant culture, primary seed culture, secondary seed culture and mixed fermentation culture, and comprises the following steps: Bacillus subtilis, Bacillus amyloliquefaciens, Lactobacillus plantarum, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces alba and Aspergillus oryzae. The microbial inoculum with high salt tolerance and oil degradation rate is compounded with 6 kinds of raw materials, which can effectively degrade the main components of kitchen waste (cellulose, oil, starch, protein), so that the kitchen waste can be fully fermented It can solve the problems of high salt content in kitchen waste, low oil degradation rate of kitchen waste, long fermentation period and poor product decay.

Description

technical field [0001] The invention relates to a microbial inoculum with high salt tolerance and oil degradation rate and a preparation method thereof. Background technique [0002] Food waste refers to food residues and wastes generated from activities such as households, catering services and unit meals. Statistics show that the proportion of kitchen waste in my country's urban domestic waste ranges from 37% to 62%. The annual amount of kitchen waste generated in major cities in China is about 60 million tons. Taking Beijing as an example, in 2014, Beijing's kitchen waste generated The amount is about 2600 tons / day. Kitchen waste is divided into two types: kitchen waste and swill: kitchen waste is the food residue produced in the process of food processing; it mainly comes from residential areas, food wholesale and retail markets and various garbage sites. Swill waste is the food residue after food consumption; it mainly comes from the catering industry, hotels and can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F11/08C05F9/04C12R1/125C12R1/07C12R1/25C12R1/865C12R1/11C12R1/465C12R1/69
CPCC12N1/14C12N1/18C12N1/20C05F9/04C05F11/08Y02A40/20
Inventor 丁国春李季王博
Owner 苏州微宏生物科技有限公司
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