A kind of GFP-2 small peptide and its preparation method and application

Abstract

The present invention relates to a GFP-2 small peptide and its preparation method and application. The small peptide is prepared by the following methods: (1) preparing a brown sugar culture medium and sterilizing it; (2) inoculating with GFP-2 bacteria, glycerol bacteria and Inoculate with LB medium and culture on a shaker; (3) Transfer to brown sugar medium, culture on a shaker, then centrifuge, collect supernatant, and discard bacteria; (4) Salt out to precipitate proteins and small peptides , centrifuge again after salting out, remove the supernatant, and collect the crude protein; (5) Resuspend the crude protein with tris‑HCl, pass the resuspended crude protein through the membrane, and dialyze with tris‑HCl; The filter tube is subjected to ultrafiltration to collect small peptides with a molecular weight of less than 10KD, and the obtained small peptides are cooled and freeze-dried to obtain GFP‑2 small peptides. The prepared small peptide can be used as an adjuvant, which can greatly improve the immune efficacy.

Description

technical field [0001] The invention relates to the technical field of biotechnology and pharmaceutical engineering, in particular to a method for preparing a small peptide adjuvant derived from marine bacillus GFP-2, and the application of the small peptide in improving the immune efficacy of vaccines. Background technique [0002] Vaccination is the most cost-effective way to prevent and treat infectious diseases, and adequate immunogenicity and safety of vaccines are the most important concerns in this field. While traditional vaccines such as live, attenuated organisms and killed or inactivated organisms are generally more immunogenic, there is a limited potential risk of reversion to virulence. Additionally, these vaccines are unstable and have low safety profiles. Compared with traditional vaccines, new vaccines such as DNA vaccines, recombinant vaccines and subunit vaccines are relatively safe. However, these vaccines also have certain defects. One of the biggest de...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the immunogenicity is often low, and the effect of the immune body cannot be achieved. To overcome the deficiency of LB medium, brown sugar medium is used to expand and cultivate GFP-2 bacteria, and a preparation of small peptide is provided. The method, the medium can increase the expression of small peptides, which is more conducive to industrial production, and the small peptides are successfully expressed and secreted into the medium, which is beneficial to the preparation and purification of small peptides, and is conducive to maintaining the activity of small peptides

Images