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Production, extraction and purification technology of DHA-enriched algae oil

A technology and technology of algae oil, which is applied in the field of production, extraction and purification of DHA-rich algae oil, can solve the problems of unsatisfactory biomass and DHA production, meet the needs of algae growth and product accumulation, and reduce the production of DHA Cost, the effect of increasing DHA production

Active Publication Date: 2018-12-21
广西小藻农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The largest biomass and DHA output; but the biomass and DHA output are not ideal, and need to be further improved to realize industrial production

Method used

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  • Production, extraction and purification technology of DHA-enriched algae oil
  • Production, extraction and purification technology of DHA-enriched algae oil
  • Production, extraction and purification technology of DHA-enriched algae oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The production, extraction and purification process rich in DHA algae oil, it comprises the following steps:

[0041] Activate the Cryptidinium ATCC30772 preserved on the slant, and then insert it into a 2L culture flask filled with 500mL shake flask seed medium for shake flask seed culture. Cultivate at 120rpm at 25°C for 48h, and then inoculate according to the inoculum size of 5%. Cultivate in a seed tank equipped with 50L seed tank culture medium, the culture conditions are: 180rpm, 25°C, ventilation rate 0.8vvm, cultivate for 48h, and obtain Cryptidinosa seed liquid, the biomass of which is 3.2g / L after detection;

[0042] The components of the shake flask seed culture medium and the seed tank culture medium are: glucose 10g / L, sodium chloride 5g / L, ammonium sulfate 2g / L, magnesium chloride 0.1g / L, potassium dihydrogen phosphate 0.1g / L ;

[0043] Inoculate the seed solution of Cryptodinium algae into the reaction tank containing the fermentation medium according t...

Embodiment 2

[0049] The production, extraction and purification process rich in DHA algae oil, it comprises the following steps:

[0050] Activate the Cryptidinium preserved on the slant, and then insert it into a 2L culture flask containing 500mL shake flask seed medium for shake flask seed culture, cultivate it at 120rpm at 25°C for 48h, and then inoculate it with an inoculum of 3%. Cultivation was carried out in the medium of the seed tank, the cultivation conditions were: 180rpm, 25°C, ventilation rate 0.5vvm, and cultivation for 72h to obtain the seed liquid of Cryptidoflagellate, with a biomass of 4.5g / L;

[0051] The components of the shake flask seed culture medium and the seed tank culture medium are: glucose 10g / L, sodium chloride 5g / L, ammonium sulfate 2g / L, magnesium chloride 0.1g / L, potassium dihydrogen phosphate 0.1g / L ;

[0052] Inoculate the seed solution of Cryptodinium algae into the reaction tank containing the fermentation medium according to the inoculum amount of 5%,...

Embodiment 3

[0058] The production, extraction and purification process rich in DHA algae oil, it comprises the following steps:

[0059] Activate the Cryptidium preserved on the slant, and then insert it into a 2L culture flask containing 500mL shake flask seed medium for shake flask seed culture, cultivate it at 120rpm at 25°C for 48h, and then inoculate it into the Cultivation was carried out in the seed tank culture medium, the cultivation conditions were: 180rpm, 25°C, ventilation rate 0.6vvm, and cultivation for 60h to obtain Cryptidinosa seed liquid with a biomass of 4.1g / L;

[0060] The components of the shake flask seed culture medium and the seed tank culture medium are: glucose 10g / L, sodium chloride 5g / L, ammonium sulfate 2g / L, magnesium chloride 0.1g / L, potassium dihydrogen phosphate 0.1g / L ;

[0061] Inoculate the seed liquid of Cryptodinium algae into the reaction tank containing the fermentation medium according to the inoculum amount of 4%, cultivate at 25°C, and the aera...

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Abstract

The invention belongs to the technical field of algae, and discloses a production, extraction and purification technology of DHA-enriched algae oil. The production, extraction and purification technology comprises the following steps of step (1) performing crypthecodinium cohnii seed culture; step (2) performing fermentation; step (3) performing extraction to obtain crude fat; and step (4) performing purification. Through adoption of the production technology disclosed by the invention, the biomass and the DHA yield of crypthecodinium cohnii can be increased; and besides, through the extraction and purification technology, the purify and the yield of the algae oil are increased, and the market application prospects are great.

Description

technical field [0001] The invention belongs to the technical field of algae, and in particular relates to the production, extraction and purification process of DHA-rich algae oil. Background technique [0002] DHA, docosahexaenoic acid, commonly known as brain gold, is an unsaturated fatty acid that is very important to the human body and is an important member of the omega-3 unsaturated fatty acid family. Animals and humans cannot synthesize DHA by themselves and must be ingested from the outside world. DHA has important physiological functions: 1) DHA is a main component of the growth and maintenance of nervous system cells, and an important component of the brain and retina. Its content in the human cerebral cortex is as high as 20%, and it accounts for the largest proportion in the eye retina. , accounting for about 50%. Therefore, it is very important for the intelligence and vision development of fetuses and babies. For a long time, most people have supplemented DHA...

Claims

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Application Information

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IPC IPC(8): C12P7/64C11B1/02C11B1/00C11B3/00C11B3/02C11B3/10C12R1/89
CPCC11B1/10C11B1/106C12N1/12C12N1/38C12P7/6427C12P7/6463
Inventor 吴善青应金良应金元夏瀚
Owner 广西小藻农业科技有限公司
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