A kind of H2S fluorescent probe based on 4-styrene pyridinium salt long-wave emission recognition and its synthesis method and application

Abstract

A method based on the long-wave emission of 4‑styrene pyridinium salts to identify H 2 S fluorescent probe and its synthetic method and application, its structural formula is as follows: preparation method is: take ethanol as solvent, 4-(diethylamino) salicylaldehyde, 4-picoline salt, piperidine carry out feeding, heating reflux reaction , the solvent was spun out, and the crude product was purified by silica gel column chromatography, and separated with methanol and dichloromethane as eluents to obtain compound 1. Using DMF as a solvent, compound 1, 2,4-dinitrofluorobenzene, and potassium carbonate were fed, stirred at room temperature, filtered to obtain a crude product, purified by silica gel column chromatography, and separated with an eluent to obtain a fluorescent Probe L. The advantages are: simple synthesis steps, easy separation and purification; fluorescent probes can recognize H 2 S, has good selectivity, high sensitivity and good anti-interference ability, and can be applied to detect H in actual water samples 2 S.

Description

technical field [0001] The invention relates to a method based on 4-styrene pyridinium salt long-wave emission recognition H 2 S fluorescent probes and their synthesis methods and applications. Background technique [0002] Hydrogen sulfide (H 2 S), a newly discovered gaseous signaling molecule, has become a research hotspot in the field of biology due to its diverse functions in physiological and pathological processes. h 2 S mainly comes from volcanic eruptions and mammalian cells themselves through cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE), 3-mercaptopyruvate sulfur transferase (3-MST), etc. produced by the action of enzymes. Previous studies have shown that H 2 S plays an important role in regulating blood pressure, cardiovascular protection, regulating cell growth and stimulating angiogenesis. In addition, high levels of H in living systems 2 S can cause human diseases such as Alzheimer's disease, Down's syndrome, hypertension and liver cirrhosi...

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Problems solved by technology

Anal. Chem., (2018), 90, 7510-7516; Anal. Chem., (2015), 87, 1188-1195; J.Org.Chem., (2017), 82, 10234-10246; Chem.Commun .,(2012),48,10871-10837; Anal.Chem.,(2013),85,7875-7881, although these documents can specifically identify H 2 S, but the emission wavelength is short, it cannot be detected in the long wavelength region, and the synthesis route is complicated and the response time is long

As we all know, in the visible spectral region, biological samples have strong background fluorescence and self-absorption, which will interfere with detection and imaging and affect the accuracy of measurement.

At the same time, the wavelength of visible light is short, and the radiation energy is relatively large during fluorescence imaging, which is easy to cause photodamage to cells and biological tissues.

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