Breast cancer typing detection kit based on illumina platform and application thereof
A detection kit, breast cancer technology, applied in the biological field to achieve the effect of disease progression and accurate and convenient control
Inactive Publication Date: 2018-12-28
江苏苏博生物医学科技南京有限公司
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In order to try to take advantage of these abundant tumor samples, researchers try to analyze the expression level by means of RT-PCR or DASL, but these methods are not capable of accurate quantification, and can only quantify a small number of known transcripts
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[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.
[0022] It should be understood that terms such as "having", "comprising", "has" and "comprising" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.
[0023] The invention belongs to the field of biological diagnosis and detection, and relates to sequencing multiple genes expressed in breast cancer FFPE samples in a high-throughput manner. More specifically, the present disclosure relates to a library construction and sequencing analysis for the sequencing of the 3' end of degraded mRNA samples, and a method for typing and risk assessment of differences in the expression profiles of breast cancer-related genes in breast cancer tissues. The detection method includes (1) extraction of total RNA in FFPE samples of tumor pati...
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The invention belongs to the field of biotechnology, and particularly relates to a breast cancer typing detection kit based on an illumina platform and an application thereof. The typing detection kitcomprises a) a degrading mRNA building reagent, primers and a magnetic bead module; b) a library quantification reagent module; c) a boarding sequencing reagent module. The primers comprise a Bio-B-TVN primer, a Primer-A random primer, a Primer-A primer, a Primer-B primer, magnetic beads are streptomycin-coated magnetic beads, and biotin on the Bio-B-TVN primer can be combined with streptomycin coated on the magnetic beads. By means of the 3' end sequencing mode, even if the obtained mRNA is partially degraded, accurate quantification of the entire transcriptome of a sample can also be achieved. Microarray for traditional application of breast cancer FFPE sample expression profile analysis and fluorescent quantification means of individual gene expression quantification cannot achieve accurate analysis while accurate quantification can be achieved by the 3' end sequencing.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for detecting the gene expression profile of breast cancer in FFPE samples to carry out molecular typing of breast cancer and its application. Background technique [0002] At present, breast cancer is still clinically based on indicators such as patient age, primary tumor size, clinical stage, histological grade, axillary lymph node metastasis, and hormone receptor status to predict the prognosis of breast cancer and guide the selection of treatment options. However, there are significant individual differences in breast cancer, and the above-mentioned criteria cannot further accurately predict the prognosis, and it is difficult to meet the "individualized" treatment plan for breast cancer. Comparative analysis of the gene expression profiles of normal breast tissue and tumors can clarify the gene expression differences at the mRNA level, so that the clin...
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IPC IPC(8): C12Q1/6886C12Q1/6869
CPCC12Q1/6869C12Q1/6886C12Q2600/158C12Q2521/107C12Q2525/191C12Q2531/113
Inventor 张吉保张明航岳伟
Owner 江苏苏博生物医学科技南京有限公司