Method for extracting phlorizin from leaf of lithocarpus polystachyus

A technology of Cordica polyphylla and phloridzin is applied in the field of extraction of flavonoids, which can solve the problems of chemical extraction and toxicity of neutral reagents, poor purification effect of chitosan, and small amount of single sample preparation, and achieves reduction of test toxicity. , The effect of low extraction equipment requirements and low prices

Active Publication Date: 2019-01-04
藤蔬生物科技(嘉兴)有限公司
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Problems solved by technology

Generally speaking, although the above methods can achieve the purpose of purification, each has its own shortcomings.
For example, the yield of PAC in the flocculation method is low and the pu

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  • Method for extracting phlorizin from leaf of lithocarpus polystachyus

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Embodiment Construction

[0034] The technical solutions of the present invention will be further described below in conjunction with specific embodiments.

[0035] In the following examples, ethanol is chromatographically pure (Tianjin East Tianzheng Fine Chemical Reagent Factory), AB-8 type macroporous adsorption resin (Donghong Chemical Co., Ltd.), polyamide resin (30-60 mesh, Shanghai Mosu Science and Technology Co., Ltd.) Ltd.).

[0036] The instruments involved in the following examples are: one-hundred-thousandth balance, SB-1100 rotary evaporator (Shanghai Ailang Instrument Co., Ltd.), SHB-Ⅲ circulating water type multipurpose vacuum pump (Zhengzhou Great Wall Technology Industry and Trade Co., Ltd., decompression pumping filter), GOLD-SIM freeze dryer and GZX electric blast drying oven (Shanghai Boxun Industrial Co., Ltd.).

[0037] In the following examples, the temperature of the rotary evaporator is 48°C.

[0038] The specification of the first chromatographic column is 25ml (volume), and...

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Abstract

The invention discloses a method for extracting phlorizin from leaf of lithocarpus polystachyus, which comprises the following steps: (1) grinding the leaf of lithocarpus polystachyus to obtain powder; (2) uniformly mixing powder with the ethanol aqueous solution, putting the mixture into a Soxhlet extractor, obtaining a first extracting solution by performing reflux extraction; (3) filtering thefirst extracting solution to obtain a filter residue and a first extracting solution filtrate, adding the ethanol aqueous solution into the filter residue, and then putting the mixture into a Soxhletextractor, obtaining a second extractive solution by performing reflux extraction; (4) mixing the first extractive solution and the second extractive solution to obtain extractive solution, concentrating the extractive solution, and to obtaining dry powder by freeze drying; (5) static adsorpting statically dry powder in a first chromatographic column and second chromatography column, flushing thesecond chromatography column by the ethanol aqueous solution, evaporating after getting second eluent, and achieving phlorizin dry powder. The method greatly shortens extraction time of organic solvent by reflux extraction of the organic solvent to obtain the phlorizin, and reduces the difficulty of anaphase purification.

Description

technical field [0001] The invention belongs to the technical field of extraction of flavonoids, and in particular relates to a method for extracting phloridzin from leaves of Aceae flavonoids. Background technique [0002] Phlorizin is the 2'-β-D-glucoside of phloretin, which is the dihydrochalcone glycoside in flavonoids. In recent years, the role of phlorizin in many fields has been discovered. In medicine, in addition to being used for the prevention and treatment of diabetes and its complications, it has been proved to have good prevention and treatment effects on osteoporosis, memory loss, obesity and cardiovascular and cerebrovascular diseases. In the cosmetic industry, phlorizin can replace arbutin as a whitening agent. It can also slow down the secretion of sebum and reduce the occurrence of acne. In addition, the sweetness of phlorizin is 300 times that of glucose, so it can be used as a natural non-sugar substitute and because it has the basic properties and fu...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 王卓龙月红邢朝斌
Owner 藤蔬生物科技(嘉兴)有限公司
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