Tissue culture rapid propagation method of curcuma alismatifolia
A technology of tissue culture rapid propagation and ginger lotus, applied in the field of plant cultivation, can solve the problems of long planting time and difficulty in forming a large number of tillers
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[0039] A kind of tissue culture rapid propagation method of lotus ginger of embodiment 1
[0040] (1) Explant preparation and disinfection:
[0041] The explant preparation and disinfection specifically include the following steps:
[0042] S11. Spray 3000 times carbendazim for sterilization every 7 days one month before the explants are collected, control the water for 6 days before the seeds are collected, the water content of the pot soil is less than 40%, and the explants are cut according to the standard; Annual new shoots, the diameter of the small buds is not more than 1cm. After cutting the single plant of the small buds, wash them under tap water while peeling off the outermost bracts, and cut off the leaves. After drying the moisture on the surface of the explant, take it into the inoculation room for surface disinfection of the explant;
[0043] S12. Soak the buds in 75% alcohol for 30 seconds, wash them twice with sterile water, and sterilize with 0.5% sodium hyp...
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