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Multi-tissue vitrification in-situ preservation device and method

A preservation device and vitrification technology, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems such as the inability to maintain the pretension force of the tissue, damage to the tissue structure, mechanical and biological properties, etc., to avoid Effect of solution damage, good biocompatibility

Active Publication Date: 2019-01-18
银丰低温医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the existing way of preserving large-scale tissues such as blood vessels is to put the tissues into low-temperature cryopreservation tubes or cryopreservation bags after being processed. The tissues cannot maintain the preload and will curl up after freezing Bending, which can cause problems such as tissue structure, mechanical and biological properties

Method used

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  • Multi-tissue vitrification in-situ preservation device and method
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  • Multi-tissue vitrification in-situ preservation device and method

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specific Embodiment 1

[0039] see figure 1 , image 3 , Figure 7 It can be seen that a multi-tissue vitrification in situ preservation device and method of the present invention comprises a preservation box 2, a cover plate 1 is arranged above the preservation box 2, and a water bath 3 is arranged below the preservation box 2; There is a fixed structure, an input structure and an output structure above the storage box 2, and the input structure is the first injection syringe 6 and the second injection syringe 7, and the first injection syringe 6 and the second injection syringe 7 are arranged between the storage box 2. There is a mixing chamber 9, the fixed structure is a carrier bracket 18, a tissue carrier is arranged above the carrier bracket 18, and a sealing structure is arranged between the cover plate 1 and the storage box 2, and the described sealing structure is the cover plate 1 There is a convex groove 13 on it, and a groove 14 around the storage box 2 that matches the convex groove 13 o...

specific Embodiment 2

[0042] see Figure 8 It can be seen that the present invention uses a multi-tissue vitrification in-situ preservation device and method, which is composed of a tissue preservation method and a tissue removal method. and extracting the protective agent and culture medium, and sealing the preservation box 2, and the method for taking out the tissue is to thaw the preservation box 2, extract the protective agent and take out the preservation tissue, and it is characterized in that the specific steps are as follows:

[0043] Step 1: Select the tissue carrier, and select the corresponding center-type carrier 4 or clamp-type carrier 5 to install on the carrier bracket 18 in the preservation box 2 according to whether the preserved tissue is blood vessel, trachea or tendon;

[0044] Step 2: Cool down the storage box 2, feed a liquid at a certain temperature into the water bath pipeline inlet 16 of the fretted water bath pipeline 15, and extract at the same speed at the water bath pip...

specific Embodiment 3

[0051] figure 1 It is a structural schematic diagram of the multi-tissue vitrification in situ preservation system provided by the embodiment of the present invention. like figure 1 As shown, the embodiment of the present invention discloses a multi-tissue vitrification in situ preservation device and method, including a cover plate 1, a preservation box 2, a water bath 3, a tissue carrier, two injection syringes with two speeds controlled separately, and a withdrawal syringe 8 and the mixing chamber 9; after the injection syringes 6 and 7 are connected in parallel, they are connected to the protective agent inlet 10; the withdrawal syringe 8 is connected to the protective agent outlet 11; the mixing chamber 9 is connected between the injection syringe and the protective agent inlet 10.

[0052] To preserve the tissue as tendon.

[0053] In the embodiment of the present application, the culture medium and protective agent solution for vitrification preservation of tendon wer...

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Abstract

The invention discloses a multi-tissue vitrification in-situ preservation device and method. The preservation comprises a preservation box, wherein a cover plate is arranged above the preservation box, and a water-bath slot is formed below the preservation box; fixed structures are arranged in the preservation box, input structures and an output structure are arranged above the preservation box, the input structures are a first injection syringe and a second injection syringe, a mixing cavity is formed among the first injection syringe, and the second injection syringe and the preservation box; and the fixed structures are carrying tool brackets, and tissue carrying tools are arranged above the carrying tool brackets. The carrying tool brackets are cooperatively arranged, and the distancebetween the two carrying tool brackets is finely adjusted by virtue of a rotary carrying tool, so that the tension of tissues is changed, and uniform concentration of solutions surrounding the tissuesin the perfusion process is guaranteed. The research on the influence of protecting agents with different concentrations on the tissue vitrification preservation result has the advantages that multiple groups of samples can be simultaneously protected, the consistency of other experiment conditions can be adequately guaranteed, and experiment results are relatively accurate and reliable.

Description

technical field [0001] The invention relates to the field of biological tissue preservation, in particular to a multi-tissue vitrification in situ preservation device and method. Background technique [0002] To maintain a healthy state of the human body, various tissues in the body need to work in harmony. Once a certain tissue becomes diseased, it will affect people's normal life and even endanger their lives. Blood vessels are the conduits for blood transport in the human body. Once damaged, blood vessels will seriously endanger the lives of patients. Blood vessel transplantation is an effective means to repair severely defective blood vessels, but the source of blood vessels is limited and the development of artificial blood vessels is not yet mature. This supply and demand greatly limits the clinical application of blood vessel transplantation. The trachea, as a pipe connecting the larynx and the bronchi, is not only a channel for air, but also has the functions of def...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0221A01N1/0226A01N1/0268
Inventor 臧传宝严震穆文杰沈凌霄刘峰李夏刘琇
Owner 银丰低温医学科技有限公司
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