Supercharge Your Innovation With Domain-Expert AI Agents!

Embryo culture solution and method for processing somatic cell cloned embryos

A technology of embryo culture medium and somatic cell cloning, which is applied in the field of cell in vitro cloning, can solve problems such as low cloning efficiency and reduced embryo reprogramming efficiency, and achieve obvious effects and simple operation

Active Publication Date: 2019-01-18
WENS FOODSTUFF GRP CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Histone deacetylase, on the other hand, can deacetylate histones, inhibit transcription, and reduce the efficiency of embryonic reprogramming.
Use fully differentiated somatic cells to construct cloned embryos. Somatic cells are oriented to transcription, and their histone deacetylase content is high, which inhibits transcriptional activation and gene expression, resulting in low cloning efficiency. Therefore, inhibition of histone deacetylation is used. Lyase is an effective means to improve cloning efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Embryo culture solution and method for processing somatic cell cloned embryos
  • Embryo culture solution and method for processing somatic cell cloned embryos
  • Embryo culture solution and method for processing somatic cell cloned embryos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] Taking pig nuclear transfer embryo construction as an example below, the present invention will be further described in detail in conjunction with the accompanying drawings.

[0024] 1. Isolation and culture of donor cells

[0025] The donor cells come from the super boar ear tissue of Guangdong Wenshi Group. The pig ear skin is cut and stored in DMEM culture medium at 4°C and transported back to the laboratory. After the pig ear skin tissue pieces are cut into pieces, the tissue fragments are washed with DMEM Resuspend with appropriate amount of fetal bovine serum and transfer to culture dish, 37°C, 5% CO 2 , cultured in a saturated humidity environment. After 5-7 hours, add DMEM culture medium containing 10% fetal bovine serum, and subculture when the cells grow to 90% confluence, and use the somatic cells passed to the second generation as donor cells for nuclear transfer.

[0026] 2. Oocyte collection and maturation culture

[0027] The ovaries obtained from sows...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses an embryo culture solution, containing 200-400 nM compound represented by structural formula (1). The blastocyst rate of cloned embryos was significantly increased by using this culture medium, thus the efficiency of cloning technology was improved. The invention also discloses a processing method of somatic cell cloned embryo, which is simple in operation and obvious in effect, and can remarkably improve the in vitro development efficiency of somatic cell nuclear transfer embryos including but not limited to porcine, cattle, sheep, mouse and human cloned embryos.

Description

technical field [0001] The invention relates to the technical field of cell in vitro cloning, in particular to an embryo culture solution and a treatment method for somatic cell cloned embryos. Background technique [0002] Although many animals have obtained adult individuals through somatic cell cloning technology, the low efficiency of this technology is still the main reason for limiting its application. Current research shows that the low efficiency is mainly caused by abnormal reprogramming of cloned embryos, such as histone hypoacetylation and DNA hypermethylation. Most of the reprogramming of the nuclei of cloned embryo donors occurs in early embryos before the embryonic genome is activated, and histone acetylation is an epigenetic modification that is one of the key factors for gene activation in embryos. After histone acetyltransferase acts on histone to acetylate it, it will lead to a loose chromatin conformation, allowing the access of transcription factors, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/14C12N2500/12C12N2500/30C12N2501/998
Inventor 吴珍芳石俊松周荣罗绿花麦然标余婉娴贺晓燕蔡更元
Owner WENS FOODSTUFF GRP CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com