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Nano-pore functionality control method and system

A control system and nanopore technology, applied in nanotechnology, material electrochemical variables, etc., can solve the problems of difficult manufacturing reproducibility, short service life of nanopores, and inability to reuse, saving time, improving efficiency, and reducing labor. The effect of the investment of resources

Active Publication Date: 2019-01-18
GUANGDONG UNIV OF EDUCATION
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  • Claims
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Problems solved by technology

[0003] To sum up, the existing problems in the existing technology are: the cost of preparing solid-state nanopores is high, the manufacturing reproducibility is difficult, the service life of nanopores is short and cannot be reused, and the control of functionality cannot be realized. Unable to meet the needs of researchers

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  • Nano-pore functionality control method and system
  • Nano-pore functionality control method and system
  • Nano-pore functionality control method and system

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Embodiment Construction

[0052] In order to further understand the content, features and effects of the present invention, the following examples are given, and detailed descriptions are given below with reference to the accompanying drawings.

[0053] The structure of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0054] Such as figure 1 As shown, the nanopore functional control system described in the embodiment of the present invention includes: a nanopore preparation module 1, a molecular construction module 2, a molecular construction solution preparation module 3, a potential application module 4, an ion current measurement module 5, and an ion current acquisition module. Module 6 , computer central processing unit module 7 , ion current data processing module 8 , construct removal module 9 , microfluid flushing module 10 , construct collection module 11 , and antibody collection module 12 .

[0055] A nanopore preparation module 1, the ...

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Abstract

The invention belongs to the technical field of nano-pore functionality, and discloses a nano-pore functionality control method and system. Manufacturing of a nano-pore is carried out through a nano-pore preparation module; preparation of molecular construction solution is carried out through a molecular construction solution preparation module; solution containing a molecular construction body isleaded to a base plate; and the molecular construction body has a main structure limiting the pore and a tail structure extending from the main structure. According to the nano-pore functionality control method and system in the invention, the potential difference is applied onto the base plate through a potential applying module, so that the tail structure passes through the nano-pore; therefore, the molecular construction body is in butt joint onto the base plate, wherein the pore is aligned to the nano-pore; the potential difference is changed through the potential applying module; and thus, the molecular construction body is removed from the base plate.

Description

technical field [0001] The invention belongs to the technical field of nanopore functionality, and in particular relates to a nanopore functional control method and system. Background technique [0002] Currently, single nanopores in insulating layers are under investigation as single-molecule sensors in solution1. Research activity in nanopore sensing has grown rapidly since the first experiments on DNA and RNA translocation by Kasianowicz et al. Single solid-state nanopores fabricated by silicon nanotechnology and ion milling have been applied for the first time in ionic current-based DNA detection. DNA folding detection using nanocapillaries, development of selective fabrication schemes such as transmission-electron drilling of solid-state nanopores, or nanopores with other single-molecule technologies such as optical tweezers, magnetic tweezers or single-molecule fluorescence, has given new impetus to the field. In a typical application, the nanopore separates two cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26B82Y40/00
CPCB82Y40/00G01N27/26
Inventor 周仁龙杨飒周桓君肖鹰翼刘丹
Owner GUANGDONG UNIV OF EDUCATION
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