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A kind of artificial cultivation method of Paecilomyces cicadae and the analgesic water extract cultivated therewith

A Paecilomyces cicadae, artificial cultivation technology, applied in the field of analgesic water extracts, can solve the problems of few research reports on analgesic activity, no analgesic effect on pain, etc., achieves great development and application prospects, and has no addiction. , the effect of obvious analgesic effect

Active Publication Date: 2021-08-17
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, related studies on Cordyceps sinensis, Paecilomyces guni, and Paecilomyces tenipedes have shown that: Cordyceps and its anamorphic fungi have obvious inhibitory effects on pain caused by chemical stimulation (peripheral analgesic effect), and have a significant inhibitory effect on pain caused by heat stimulation. Pain does not show analgesic effect (central analgesic effect), and there is no report of addiction; and there are few reports on the analgesic activity of Paecilomyces cicadae mycelium, anamorph of cicadae

Method used

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  • A kind of artificial cultivation method of Paecilomyces cicadae and the analgesic water extract cultivated therewith
  • A kind of artificial cultivation method of Paecilomyces cicadae and the analgesic water extract cultivated therewith
  • A kind of artificial cultivation method of Paecilomyces cicadae and the analgesic water extract cultivated therewith

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Experimental program
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preparation example Construction

[0033] Preparation of Paecilomyces cicadae aqueous extract:

[0034] (1) Get the artificially cultivated Paecilomyces cicadae fermentation product, 4000r / min, centrifuge 10-20min, abandon the supernatant, get Paecilomyces cicadae mycelium;

[0035] (2) Broken Paecilomyces cicada mycelium cells with a glass homogenizer, according to the ratio of mycelium (kg) to ultrapure water (L) 1:4-1:10, at room temperature, add ultrapure water to extract 3-5 times;

[0036] (3) The above water extract is centrifuged at 4000r / min for 10-20min, and the supernatant is taken;

[0037] (4) the supernatant is subjected to SephadexG-25 gel column chromatography to obtain the active component;

[0038] (5) The water extract is formulated into an aqueous solution containing 0.1-1.0 mg / ml of the active component, which is the Paecilomyces cicadae analgesic aqueous solution of the present invention.

Embodiment 1

[0039] Embodiment 1 (best parameter):

[0040] (1) artificially cultivated Paecilomyces cicadae fermentation product, 4000r / min, centrifugal 10-20min, abandon supernatant, obtain Paecilomyces cicadae mycelia 10g;

[0041] (2) Broken Paecilomyces cicadae mycelia cells with a glass homogenizer, according to mycelium (kg) and pure water (L) ratio 1:8, add 80ml pure water, at room temperature, add ultrapure water leaching 5 Second-rate;

[0042] (3) The above-mentioned water extract is centrifuged at 4000r / min for 10min, and the supernatant is taken;

[0043] (4) The supernatant was subjected to SephadexG-25 gel column chromatography to obtain the active components; the active components were shown in the gel column chromatogram as shown in Figure 1.

[0044] (5) The aqueous extract is formulated into an aqueous solution containing 0.40 mg / ml ± 0.02 mg / ml of the active component. (The concentration is determined by the Folin phenol method specified in the Chinese Pharmacopoeia)...

Embodiment 2

[0046] (1) artificially cultivated Paecilomyces cicadae fermentation product, 4000r / min, centrifugal 10-20min, abandon supernatant, obtain Paecilomyces cicadae mycelia 10g;

[0047] (2) Broken Paecilomyces cicadae mycelial cells with a glass homogenizer, according to mycelium (kg) and pure water (L) ratio 1:4, add 40ml pure water, at room temperature, add ultrapure water leaching 3 Second-rate;

[0048] (3) The above-mentioned water extract is centrifuged at 4000r / min for 20min, and the supernatant is taken;

[0049] (4) The supernatant is subjected to SephadexG-25 gel column chromatography to obtain the active component; the gel column chromatogram is approximate as shown in Figure 1;

[0050](5) The aqueous extract is formulated into an aqueous solution containing 0.40 mg / ml ± 0.02 mg / ml of the active component.

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Abstract

An artificial cultivation method of Paecilomyces cicadae, comprising the following steps: (1) Strain activation: Paecilomyces cicadae GZUIFR04606 with strong vitality and high production of analgesic and metabolic active substances was isolated and bred from nature, and the strain was inoculated On the slant of the PDA test tube, culture at 25°C for 7 days; (2) Seed culture: Use an inoculation loop to inoculate the activated bacteria on the slant of the test tube into a 250mL Erlenmeyer flask containing 50mL of liquid medium, at 25°C, 150r / min , shaking culture on a shaking table for 72-96 hours; (3) Fermentation culture: Inoculate the seed culture solution into a 500mL Erlenmeyer flask containing 150mL liquid medium according to the inoculum amount of 5% (7.5ml), at 25°C, 150r / min, Shaker shaking culture 72 ~ 96h. The Paecilomyces cicadae is used as a raw material, homogenized with pure water, centrifuged, and subjected to gel column chromatography to obtain an analgesic aqueous extract of the Paecilomyces cicadae with active components. The analgesic aqueous extract of Paecilomyces cicadae has obvious analgesic effect, little toxic and side effects, good stability and no addiction.

Description

technical field [0001] The invention belongs to the technical field of fermentation of Cordyceps fungus, extraction of effective parts and utilization thereof, and in particular relates to an artificial cultivation method of Paecilomyces cicadae and the analgesic water extract cultivated therein. Background technique [0002] Paecilomycescicadae is the conidia anamorph stage of Cordycepscicadae S-hing. Cicada is a nymph of cicada in the soil that is parasitized to death by Paecilomyces cicadae. The spore bundles formed by mycelium in its head are like flower buds, so it is called cicada. Because of the geographical location and ecological environment where cicada grows Different, cause cicadae to have different shapes. Cicadae is regarded as one of the "Eight Treasures of Chinese Medicine", and it has a long history of being used as a medicinal material in my country. It has been recorded in "Lei Gong Pao Shao Lun" written by Lei Xiao in the Southern and Northern Dynasties....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/068A61P29/00C12R1/79
CPCA61K36/068A61K2236/331A61K2236/55A61P29/00C12N1/14
Inventor 谭艾娟陈波利吕世明钱文丹刘爱英邹晓
Owner GUIZHOU UNIV