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Method for regulating nerve cell growth by using polyvinyl alcohol/silk fibroin hydrogel

A technology of polyvinyl alcohol and silk fibroin, applied in nervous system cells, tumor/cancer cells, biochemical equipment and methods, etc., can solve the problem of no nerve cells, etc., achieve strong operability, wide application and promotion prospects Effect

Active Publication Date: 2021-12-10
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are reports about PVA / Silk hydrogel in the existing literature, but there is no research on the influence of PVA / Silk hydrogel on the growth of nerve cells, such as Zhang Dekun et al. friction properties; Zhou Qiqi et al. mixed sheep vertebral powder and nano-hydroxyapatite into PVA / Silk hydrogel to study bone defect repair

Method used

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  • Method for regulating nerve cell growth by using polyvinyl alcohol/silk fibroin hydrogel
  • Method for regulating nerve cell growth by using polyvinyl alcohol/silk fibroin hydrogel
  • Method for regulating nerve cell growth by using polyvinyl alcohol/silk fibroin hydrogel

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Embodiment 1

[0023] The polyvinyl alcohol / silk fibroin hydrogel of embodiment 1 regulates the method for nerve cell growth, comprises the following steps:

[0024] (1) Weigh 20g of PVA, add 250mL of deionized water, 90°C water bath, magnetically stir for 1.5h until the PVA is completely dissolved, then cool to room temperature to obtain a PVA solution with a concentration of 8% by mass; weigh 50g of 8% PVA PVA solution, 0.93gSilk is added in this PVA solution subsequently, obtains PVA / Silk solution after stirring for 2h;

[0025] (2) Take 1mL of PVA / Silk solution with a disposable pipette, drop it into three 48-well cell culture plates respectively, and freeze-thaw the three 48-well cell culture plates for 1 time, 2 times and 6 times respectively. The freezing temperature of each freezing-thawing cycle is -20°C, the freezing time is 16h, the thawing temperature is 25°C, and the thawing time is 8h. PVA / Silk hydrogels with 1, 2, and 6 cycles were prepared respectively. , referred to as PS1,...

Embodiment 2

[0027] The polyvinyl alcohol / silk fibroin hydrogel of embodiment 2 regulates the method for nerve cell growth, comprises the following steps:

[0028] (1) Weigh 20g of PVA, add 250mL of deionized water, 90°C water bath, mechanically stir for 1.5h until the PVA is completely dissolved, then cool to room temperature to obtain a PVA solution with a mass percentage concentration of 8%; weigh 50g of 8% PVA PVA solution, 0.93gSilk is added in this PVA solution subsequently, obtains PVA / Silk solution after stirring for 2h;

[0029] (2) Take 1mL of PVA / Silk solution with a disposable pipette, drop it into three 48-well cell culture plates respectively, and freeze-thaw the three 48-well cell culture plates for 1 time, 2 times and 6 times respectively. The freezing temperature of each freezing-thawing cycle is -20°C, the freezing time is 16h, the thawing temperature is 25°C, and the thawing time is 8h. PVA / Silk hydrogels with 1, 2, and 6 cycles were prepared respectively. , referred to...

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Abstract

The invention discloses a method for regulating the growth of nerve cells by using polyvinyl alcohol / silk fibroin hydrogel. The number of melting cycles was used to construct polyvinyl alcohol / silk fibroin hydrogels with different elastic moduli, and the growth of nerve cells was regulated by the polyvinyl alcohol / silk fibroin hydrogels. The method of the present invention has strong operability, does not add any growth factors, does not adopt cumbersome and complicated design routes, and only changes the elastic modulus of polyvinyl alcohol / silk fibroin hydrogel in a simple and easy way to effectively regulate neurons. Cell growth has broad application and promotion prospects in the field of biomedicine.

Description

technical field [0001] The invention relates to the field of hydrogel and biomedical technology, in particular to a method for regulating nerve cell growth by using polyvinyl alcohol / silk fibroin hydrogel. Background technique [0002] Hydrogel has a three-dimensional network structure, can absorb a large amount of water to swell but not dissolve, and has a structure similar to extracellular matrix, which is one of the ideal alternative materials in the field of biomedicine. [0003] Polyvinyl alcohol (PVA) hydrogel has high mechanical properties, lubricity, and good biocompatibility, but it is not conducive to cell growth. Silk fibroin (Silk) is a natural high-molecular fiber protein extracted from silk, containing a variety of amino acids, which is conducive to cell adhesion, proliferation and growth. However, silk fibroin alone as a hydrogel has poor stability, low mechanical strength, and poor swelling performance. Therefore, it is a better choice to combine PVA and Si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/079C12N5/09C08J3/075C08L29/04C08L89/00
CPCC12N5/0622C12N5/0693C08J3/075C08J2329/04C08J2489/00C12N2533/30C12N2533/50
Inventor 侯瑞霞竺亚斌王毓茜韩佳丽张立兵李丽萍
Owner NINGBO UNIV
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