A miRNA prediction method, a miRNA system and applications

A prediction method and seed technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of long running time, increase the consumption of computing resources, affect the project cycle, etc., and achieve high efficiency.

CN109280698AInactive Publication Date: 2019-01-29BGI TECH SOLUTIONS

9 Cites 0 Cited by

Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: BGI TECH SOLUTIONS
Publication Date: 2019-01-29
Estimated Expiration: Not applicable · inactive patent

Smart Images

Figure 1

Patent Text Reader

Abstract

The invention discloses a miRNA prediction method, a miRNA system and applications. The miRNA prediction method of the invention includes the following steps: (1) setting a length of a seed sequence;(2) fully arranging four bases A, T, C and G according to the set length to form a seed sequence set; (3) removing redundant sequences in the seed sequence set; (4) searching positions of the seed sequences on a genome to obtain a position set; (5) arranging the seed sequence position set from small to large; (6) traversing the positions of the position set, and if the sequences of the front and back positions complement each other and the distance is not greater than the length of the set precursor sequence, outputting the sequence; and (7) using secondary structure prediction software to perform secondary structure prediction on the output sequence to obtain miRNA, and generating a miRNA set of the species. The miRNA prediction method of the invention directly searches for the miRNA in the genome sequences, and so the obtained miRNA set is more complete. Furthermore, the miRNA prediction method is high in efficiency and more convenient, and lays the foundation for subsequent miRNA research.

Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present application relates to the field of nucleic acid analysis and detection, in particular to a miRNA prediction method, a miRNA system obtained by the miRNA prediction method, and the application of the miRNA prediction method. Background technique

[0002] miRNA is a kind of non-coding single-stranded RNA molecule encoded by endogenous genes with a length of about 22 nucleotides. It regulates gene expression through complete or incomplete complementary combination with genes, thereby causing differences in shape and phenotype. At present, there are many miRNA-related databases, such as miRBase, Tarbase, etc., which are conducive to downstream analysis of miRNA. However, except for a small number of model organisms, the number of known miRNAs in most species is very limited. For example, only 173 miRNAs are included in miRBase for amphioxus, which limits further research on miRNAs to some extent. Therefore, for The identification of potential...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More