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A nscqds/bi based 2 the s 3 Photoelectrochemical sensor and preparation method thereof

A photoelectrochemical and sensor technology, used in scientific instruments, instruments, material analysis by electromagnetic means, etc., can solve problems such as weak light absorption, achieve low detection limit, wide detection range, and promote separation.

Active Publication Date: 2020-11-27
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But Bi 2 S 3 The visible light absorption range is 300-510nm, and the absorption of 510-800nm ​​light is weak

Method used

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  • A nscqds/bi based <sub>2</sub> the s <sub>3</sub> Photoelectrochemical sensor and preparation method thereof
  • A nscqds/bi based <sub>2</sub> the s <sub>3</sub> Photoelectrochemical sensor and preparation method thereof
  • A nscqds/bi based <sub>2</sub> the s <sub>3</sub> Photoelectrochemical sensor and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A NSCQDs / Bi-based 2 S 3 The preparation of photoelectrochemical sensors, such as figure 1 As shown, it mainly includes the following steps:

[0047] 1. Bi 2 S 3 Preparation of nanorods

[0048] Preparation of Bi by hydrothermal method 2 S 3 Nano stave. Mainly include: weigh 1.820g of Bi(NO 3 )·5H 2O in 25mL of ethylene glycol, after stirring for 10min, a transparent solution A was formed. Weigh 1.325g of Na 2 S·9H 2 O was placed in 15mL ethylene glycol and 15mL deionized water, and stirred for 10min to form a transparent solution B. Under strong stirring conditions, solution B was added dropwise to solution A, resulting in a large amount of black suspension. Then, 1.922 g of urea and 15 mL of deionized water were added to the above solution, stirred vigorously for 20 minutes, then transferred to a polytetrafluoroethylene reactor, and the reactor was placed at 180° C. for 24 hours. After the reaction was completed, the reaction kettle was naturally cooled t...

Embodiment 2

[0084] The NSCQDs / Bi prepared by the above method 2 S 3 The photoelectrochemical sensor used for the detection of ATP mainly includes: taking 8 μL of AFP solutions with different concentrations and adding them dropwise to BSA / Ab / NSCQDs / Bi 2 S 3 / ITO electrode surface, after incubation at room temperature for 1 h, the electrode was washed with PBS (0.1M, pH=7.4) solution. The obtained immunosensing electrode was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum wire electrode was used as the counter electrode, and the photocurrent signal was tested in a solution of PBS (0.1M, pH=7.4) containing 0.15M AA. Under the condition of no bias voltage, the excitation light is filtered by a filter (λ≥420nm), and the light intensity is 20mW cm -2 , and implement light / shield light at intervals of 10s, and conduct photocurrent test.

[0085] Different concentrations of AFP(a-h)0.001, 0.01, 0.1, 1.0, 10, 100, 300, 500ng·mL -1 The...

Embodiment 3

[0093] In order to make the prepared photoelectrochemical bioimmunosensor exhibit the best performance, the relevant parameters of the preparation process were optimized as follows.

[0094] During the construction of photoelectrochemical biosensors, Bi 2 S 3 The concentration of the sensor plays a very important role in the performance of the sensor. Such as Figure 12 As shown in (A), with Bi 2 S 3 Concentration from 0.5mg·mL -1 Increased to 3.0mg·mL -1 , Bi 2 S 3 / ITO electrode photocurrent increases gradually; continue to increase Bi 2 S 3 Concentration up to 7 mg·mL -1 When Bi 2 S 3 / ITO electrode photocurrent began to decline gradually. This is because with Bi 2 S 3 With the increase of the concentration, the Bi formed on the electrode surface 2 S 3 The layer will become thicker, which will not only hinder the transfer of electrons, but also increase the recombination probability of photogenerated electron-hole pairs, thus reducing the output photocurren...

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Abstract

The invention relates to a photo-electrochemical sensor based on NSCQDs / Bi2S3 and preparation and application thereof. A Bi2S3 nanorod serves as a photoactive material, NSCQDs synthesized through a hydrothermal method is subjected to electrostatic adsorption through PDDA; then carboxy on the surface of the NSCQDs is activated through EDC and NHS, and through an amidation reaction, Ab is modified on the electrode surface; and in the presence of AFP, through antigen-antibody recognition, and an Ab-AFP immune complex is formed on the electrode surface, so that steric hindrance on the electrode surface is increased, transmission of electronic sacrificial agent ascorbic acid (AA) to the electrode surface is hindered, and consequently, photoelectric current signals of the sensor are changed. According to the relation between the AFP concentration and the sensor photoelectric current, the AFP concentration is detected; the photo-electrochemical sensor has good selectivity, repeatability and stability; detection operation on ATP is easy, the cost is low, high efficiency and high sensitivity are achieved, and the photo-electrochemical sensor has the low detection limit and the wide detection range and is of important significance to clinical and biological analysis of proteins.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical sensor detection, in particular to a method based on NSCQDs / Bi 2 S 3 Photoelectrochemical sensor and its preparation and application. Background technique [0002] With the progress and development of society, human beings are more and more concerned about environmental protection and health and safety issues. Currently, cancer is one of the major threats to human beings and their health. Therefore, the detection of cancer markers plays a very important role in early screening and disease diagnosis. Alpha-fetoprotein (AFP) is a human plasma protein encoded by the AFP gene, produced by the yolk sac and liver during fetal life. AFP is widely considered as a cancer marker. If there is a high concentration of AFP in adult plasma, it may be related to the occurrence of certain cancers, such as hepatocellular carcinoma, endodermal carcinoma, testicular carcinoma, and teratogenesis canc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3277G01N27/3278
Inventor 高文华成文杰陈耀文张晓珊林月娟徐严平陈图锋
Owner SHANTOU UNIV
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