Fully automatic micro-droplet emulsion generating device and generating method

A technology for generating devices and microdroplets, applied in the field of microfluidics, can solve the problems of sample loss, waste of channel use, and inability for users to know, and achieve the effect of isolating sample contamination and saving reagent usage.

Pending Publication Date: 2019-02-01
SUZHOU PRECIGENOME LTD CO
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  • Abstract
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  • Claims
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Problems solved by technology

At the same time, BioRad's emulsion generation microfluidic chip has 8 channels (emulsion preparation of 8 samples can be performed at the same time), but their system must do 8 channels at the same time. If the user has less than 8 samples, The user must fill the remaining channels with oil or buffer, resulting in wasted channel use
If the w

Method used

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  • Fully automatic micro-droplet emulsion generating device and generating method
  • Fully automatic micro-droplet emulsion generating device and generating method
  • Fully automatic micro-droplet emulsion generating device and generating method

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Embodiment Construction

[0035] The technical solutions of the present invention are described in detail below in conjunction with the examples. The present invention discloses a fully automatic micro-droplet emulsion generating device and a generating method thereof. combine Figure 1-Figure 4 As shown, the device includes a base 2, a support plate 3 vertically arranged on the base, a slide rail 6 is arranged on the base 2, and a droplet generation chip is mounted on the slide rail 6. Specifically, a tray 8 is arranged on the slide rail 6 , the droplet generation chip 83 is arranged on the tray 8 through a chip holder 81 , and one side of the tray 8 is connected to a driving motor 82 . The movement of the drive motor 82 drives the droplet generation chip 83 to move horizontally on the slide rail 6 . The driving motor 82 is connected to the chip holder through a connecting rod, and one end of the driving motor 82 is disposed on the support plate 3 . Of course, the driving motor 82 can also be fixed ...

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Abstract

The invention provides a fully automatic micro-droplet emulsion generating device and generating method, wherein the device includes a base and a support plate; a slide rail is arranged on the base, amicro-droplet generating chip is arranged on the slide rail through a chip fixture frame, one side of the chip fixture is connected with a driving motor, and the driving motor moves to drive the chipfixture to move on the slide rail; one side of the support plate is provided with a guide rail, and an air pressure control module is arranged on the guide rail; an air cylinder is arranged above theguide rail, and an air cylinder shaft of the air cylinder is connected with a connection plate; the air cylinder moves, and the air pressure control module is driven to move on the guide rail. The reaction conditions of droplet formation can be adjusted by automatic configuration, so that micro-droplets with different sizes are produced, and the needs of different application occasions are met; sample pollution is isolated by a sealing cover, and a microdroplet generation chip is protected from being damaged by a press plate during operation. The eight-channel isolation system realizes preparation of droplets in two or multiple channels, is more convenient than conventional equipment and saves the use amount of reagents.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and in particular relates to a fully automatic micro-droplet emulsion generating device and a generating method thereof. Background technique [0002] Micro-emulsions formed from oil-water phases have been used in high-throughput analysis systems, and their application range has a tendency to expand. For example, emulsion formation is used in combination with polymerase chain reaction (PCR) to amplify a single copy of a nucleic acid target molecule in a droplet, thereby realizing the detection of the presence of a single molecule target and its gene sequence. 10X Genomics, an American company, uses the emulsion PCR method combined with GemCode technology to generate long-chain DNA, thereby realizing long-chain DNA sequence sequencing, or single-cell sequencing, which has been recognized by the industry as one of the best technologies for long-chain DNA sequencing and single-cell sequencing ...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L2200/0689B01L2300/0809B01L2400/06
Inventor 凌云峰李琛房词峰刘宇张华王雅琦
Owner SUZHOU PRECIGENOME LTD CO
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