Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paenibacillus strain producing post-sun repairing polysaccharide and application thereof

A Bacillus sp., post-sun repair technology, applied in bacteria, cosmetic preparations, skin care preparations, etc. Active ingredients are easily available and inexpensive

Active Publication Date: 2020-05-29
ZHEJIANG UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, UV may induce cells to produce excessive reactive oxygen species (ROS), destroy their own antioxidant defense system, cause lipid peroxidation, affect related signal transduction pathways, damage cell structure or function, and induce immunosuppression, malignant tumors, etc. disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paenibacillus strain producing post-sun repairing polysaccharide and application thereof
  • Paenibacillus strain producing post-sun repairing polysaccharide and application thereof
  • Paenibacillus strain producing post-sun repairing polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Example 1: Extraction and content determination of exopolysaccharides produced by the above strains

[0023] An appropriate amount of the above strains is picked from the sugar-producing solid medium and inoculated into the sugar-producing fermentation medium. At 30°C, shake culture at 150 rpm for 48 hours to obtain a fermentation broth. figure 1 It is the plate culture trait of the bacterial strain PYQ1 of the present invention.

[0024] Among them, the used sugar-producing solid medium components are: sucrose 20g / L, tryptone 10g / L, yeast powder 5g / L, Na 2 HPO 4 12H 2 O 3g / L, agar 20g / L, and the balance is distilled water. The components of the sugar-producing fermentation broth used are: sucrose 50g / L, tryptone 5g / L, yeast powder 1g / L, Na 2 HPO 4 12H 2 O 3g / L, the balance is distilled water.

[0025] The above fermentation broth was centrifuged at 4000 g for 10 min to remove the bacteria to obtain the supernatant. Add 1 / 4 volume of Sevage solution (n-butanol:...

example 2

[0027] Example 2: Identification of microbial strains of the present invention

[0028] Such as figure 2 The strain shown is purple by Gram stain and is Gram-positive. According to the steps of TakaraBacteria Genomic DNA Extraction Kit (TakaraBacteria Genomic DNA Extraction Kit) to extract the total DNA of the strains described in the present invention, amplify the 16S rDNA gene of the bacteria with the general primers 27F and 1492R, recover the amplified products and carry out Sequencing, identification of bacterial species by determining the sequence of 16S rDNA. The obtained sequence results were compared by Blast at NCBI, and the recognized standard sequence data homologous to the 16S rDNA of the strain was obtained from the GenBank database. The sequence similarity was calculated using MEGA software and the Neighbor-Joining algorithm (Neighbor-Joining) was used as the system developmental analysis.

[0029] The phylogenetic tree of the constructed strain is as follows...

example 3

[0030] Example 3: Use of the above-mentioned polysaccharides as a repairing agent for sun damage

[0031] HaCaT cells in the logarithmic phase were collected and inoculated in a 96-well plate with 5000 cells per well, and cultured in a 37°C, 5% CO2 incubator. Cells were divided into control group, UV model group, polysaccharide group (1mg / mL), octocrylene group (8%) and Vc group (1mg / mL), wherein the latter two groups were positive controls, and each group repeated 3 wells. After the cells were cultured for 24 hours, the culture medium was replaced with phosphate buffered saline (PBS) and irradiated with UV light, and the control group was wrapped with tinfoil to avoid light. Aspirate the PBS and add the culture medium, wherein the culture medium of the polysaccharide group contains 1 mg / mL of the prolific exopolysaccharide of the above strains, and the culture medium of the octocrylene group contains 8% 2-cyano-3,3-di Isooctyl phenylacrylate, Vc group medium contains 1mg / mL ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Paenibacillus bacterial strain producing post-sun repairing polysaccharide and application thereof. The strain is classified as Paenibacillus polymyxa PYQ1, and is preserved in China General Microorganism Culture Collection and Management Center with the preservation number CGMCC No.16444. The invention includes the isolation and cultivation of the bacterial strain and the identification of 16S rDNA; the acquisition of the extracellular polysaccharide of the fermentation product of the bacterial strain and the application of the extracellular polysaccharide produced by the bacterial strain in repairing sun damage. The polysaccharide produced by Paenibacillus PYQ1 disclosed by the present invention can significantly improve the value-added activity of sun-damaged cells, and has obvious sun-damage repairing effects. As a natural product of microbial fermentation, it has the characteristics of low cost, simple and easy to obtain, and can be applied It is used in the preparation of related after-sun repair agents, and has a good market application prospect.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and microorganism technology, and particularly relates to a Bacillus strain capable of producing exopolysaccharide with after-sun repairing effect and its application. Background technique [0002] According to different wavelengths, ultraviolet rays can be divided into three categories: long-wave ultraviolet (UVA) with a wavelength of 320-400nm, medium-wave ultraviolet (UVB) with a wavelength of 280-320nm, and short-wave ultraviolet (UVC) with a wavelength of 200-280nm. The ozone layer located more than ten kilometers above the earth's surface can absorb all the UVC and most of the UVB, which is the most harmful to human skin, and is a natural barrier for biological life activities on the earth. However, with the increase of air pollution year by year, the ozone layer has been severely damaged, and the ultraviolet rays radiated to the earth's surface have increased significantly, af...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04A61K8/73A61Q19/00C12R1/01
CPCA61Q19/004A61K8/73C12N1/20C12P19/04C12R2001/01C12N1/205
Inventor 周文文汪旖清
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com