Multi-color super resolution microscope system with automatic alignment function

A microscope system and automatic alignment technology are applied in the field of multi-color super-resolution microscope systems to achieve the effect of low difficulty and low cost

Active Publication Date: 2019-02-19
ZHEJIANG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, since the wave plate itself is not an optical device with wavelength selective characteristics, in order to minimize the influence on the excitation light, the wavelength of the excitation light can only be limited to a narrow range according to the dispersion curve of the wave plate
Therefore, the easySTED architecture is only suitable for monochromatic super-resolution microscopy

Method used

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  • Multi-color super resolution microscope system with automatic alignment function
  • Multi-color super resolution microscope system with automatic alignment function
  • Multi-color super resolution microscope system with automatic alignment function

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Experimental program
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Embodiment

[0034] see Figure 1 to Figure 6 , the multi-color super-resolution microscope system with automatic alignment function of this embodiment includes:

[0035] Excitation light source 1, suppression light source 2, first light modulator 3, second light modulator 4, relative phase delay component 5, beam combining element 6, first 1 / 2 wave plate 7, first lens 8, single Mode polarization maintaining fiber 9, second lens 10, second 1 / 2 wave plate 11, phase modulation assembly 12, first dichroic mirror 13, first telescopic assembly 14, scanning assembly 15, second telescopic assembly 16, The first mirror 17, the first 1 / 4 wave plate 18, the microscope objective lens 19, the sample stage 20, the imaging component 21 and the computer 22. All optical components and fluorescent samples are located on the coaxial optical path.

[0036] In this embodiment, the excitation light beam from the excitation light source 1 reaches the beam combining element 6 after passing through the first li...

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Abstract

The invention discloses a multi-color super resolution microscope system with an automatic alignment function, belonging to the field of microscopic imaging. The system couples an excitation beam anda suppression beam into the same single mode polarization-maintaining fiber to form a mixed beam. Only the suppression beam is affected while the excitation beam is unaffected when the mixed beam is phase modulated via a phase modulation assembly. According to the multi-color super resolution microscope system with the automatic alignment function, the influence of beam drift on the performance ofthe super resolution microscope system can be avoided, so that the system structure is simplified, and the system stability is improved. Compared with the prior art, the system disclosed by the invention can conveniently realize multi-color imaging.

Description

technical field [0001] The invention relates to the field of microscopic imaging, in particular to a multi-color super-resolution microscope system with an automatic alignment function. Background technique [0002] Like most optical imaging, the Abbe diffraction limit has restricted the improvement of the resolution of the microscope system since the invention of the microscope. Early microscope systems were all wide-field imaging systems with limited imaging resolution. This situation was not improved to some extent until the invention of the confocal microscope system (Confocal Microscope). The basic concept of confocal microscopy was proposed by Marvin Minsky in 1957 (see Microscopy Apparatus, US Patent US3013467A), but the technique was not truly instrumented until 1978 (see Christoph Cremer et al. microscope with high resolution and depth of field", Microscopia Acta 81, 31-44(1978)). Compared with the traditional wide-field microscope system, the confocal microscope...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N21/643G01N21/6445G01N21/6458G01N2021/6439
Inventor 郝翔涂世杰刘鑫刘旭
Owner ZHEJIANG UNIV
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