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A kit for liver cancer detection

A kit and technology for liver cancer, applied in the field of medical testing, can solve the problems of inability to effectively determine the degree of cancer invasiveness, the significance of diagnosis is not large, and the detection rate of missed diagnosis is high, so as to achieve rapid, sensitive and effective screening judgment and save detection. Cost, simple effect of specimen

Active Publication Date: 2021-11-02
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing kits for detection of liver cancer markers are of little significance in the early diagnosis of cancer. After positive results, the cancer has often developed to the middle and late stages, and the degree of cancer invasion cannot be effectively determined. Moreover, the detection rate of missed diagnosis is relatively low. Big
Therefore this area still lacks very effective test kit

Method used

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  • A kit for liver cancer detection
  • A kit for liver cancer detection
  • A kit for liver cancer detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The structure of the kit is as follows: it is equipped with a box body, an ELISA plate located in the box body and a reagent bottle; specifications: 48T / 96T, boxed liquid. The kit includes two parts A1-6 and B1-5:

[0047] 1. A1-6 Reagent part structure and content:

[0048] 1) Buffer solution (PBS): 8g sodium chloride, 0.2g potassium chloride, 2.7g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, add distilled water to 1000ml, pH: 7.0-7.4.

[0049] Dilution of 20× buffer: distilled water is diluted 1:20, that is, 1 part of 20× buffer plus 19 parts of distilled water.

[0050] 2) HRP-labeled LPA antibody: (imported finished product).

[0051] 3) Lysophosphatidic acid (lysophosphatidic acid, LPA) standard substance and negative control substance. (Standard products are imported finished products)

[0052] 4) Washing solution (PBST): PBS solution containing 0.05% Tween-20 (500 μl Tween-20 was added to 1 LPBS and mixed well).

[0053] 5) TMB chromogen...

Embodiment 2

[0064] 1. Part A1-6 testing:

[0065] 1. Take out the required slats from the bag after equilibrating at room temperature for 20 minutes.

[0066] 2. Mark the standard wells, sample wells and blank wells, and add 50 μL of standard substances of different concentrations to each standard well;

[0067] 3. Add 50 μL of the sample to be tested into the sample well; do not add to the blank well.

[0068] 4. Add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each well of standard wells and sample wells, seal the reaction wells with plate sealing film, and incubate for 60 min in a 37°C water bath or incubator.

[0069] 5. Discard the liquid, pat dry on absorbent paper, fill each well with washing solution (350 μL), let it stand for 1 min, shake off the washing solution, pat dry on absorbent paper, and repeat washing the plate 5 times (or wash the plate with a plate washer) .

[0070] 6. Add 50 μL each of substrate A and B to each well, and incubate at 37°C in...

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Abstract

The invention relates to the field of medical testing, in particular to a kit for liver cancer detection. The kit of the invention has the characteristics of convenience, quickness, sensitivity and the like, because of its stable property, convenient use and low price. Through the detection of purine metabolites and phospholipid metabolites, not only can rapid, sensitive and effective screening and judgment of liver cancer be achieved, while reducing the rate of missed diagnosis, it can also detect the degree of invasion of liver cancer in two directions. Risk classification has great clinical application value. Through the detection, it is of great guiding significance to assist in disease risk assessment, so as to make early diagnosis of the above-mentioned patients; to observe the efficacy of medication or surgery, and to monitor the recovery degree of liver cancer treatment; and to provide simple, more economical, safe and effective detection of specimens.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a kit for liver cancer detection. Background technique [0002] Liver cancer is the most common malignant tumor in China, and its incidence is still on the rise in recent years. The prognosis of patients with early diagnosis is good, and the 5-year survival rate after operation is improved. Liver cancer refers to malignant tumors that occur in the liver, including primary liver cancer and metastatic liver cancer. People usually refer to primary liver cancer when they say liver cancer. Primary liver cancer is one of the most common malignant tumors in clinical practice. According to the latest statistics, there are about 800,000 new liver cancer patients in the world every year, ranking fifth among malignant tumors. According to cell type, primary liver cancer can be divided into hepatocellular carcinoma, cholangiocellular carcinoma and mixed liver carcinoma. [0003] The diagnos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/52
CPCG01N33/52G01N33/57438
Inventor 王若雨王亚东张晓辉周士胜
Owner DALIAN UNIV
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